Isolation, characterization and molecular cloning of a leaf-specific lectin from ramsons (Allium ursinum L.). NLM AIDSLINE Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.

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Isolation, characterization and molecular cloning of a leaf-specific lectin from ramsons (Allium ursinum L.).

Plant Mol Biol. 1997 Nov;35(4):531-5. Unique Identifier : AIDSLINE /MED98009988
Smeets K; Van Damme EJ; Van Leuven F; Peumans WJ; Laboratory for Phytopathology and Plant Protection, Katholieke; Universiteit Leuven, Belgium.

Abstract: Lectins were isolated from roots and leaves of ramsons and compared to the previously described bulb lectins. Biochemical analyses indicated that the root lectins AUAIr and AUAIIr are identical to the bulb lectins AUAI and AUAII, whereas the leaf lectin AUAL has no counterpart in the bulbs. cDNA cloning confirmed that the leaf lectin differs from the bulb lectins. Northern blot analysis further indicated that the leaf lectin is tissue-specifically expressed. Sequence comparisons revealed that the ramsons leaf lectin differs considerably from the leaf lectins of garlic, leek, onion and shallot.
Keywords: *Allium/CHEMISTRY *Lectins/GENETICS *Lectins/ISOLATION & PURIFKWDallium/chemistryKWDlectins/geneticsKWDlectins/isolation&purif
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M9810902

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