Mouse mammary tumor virus (MMTV) antigens expressed in nuclei of variant lymphoma cells (Meeting abstract). NLM AIDSLINE Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.

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Mouse mammary tumor virus (MMTV) antigens expressed in nuclei of variant lymphoma cells (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 38:A1058 1997. Unique Identifier : AIDSLINE ICDB/98638058
Hoch-Marchaim H; Hasson T; Rorman E; Braun E; Hochman J; Hebrew University of Jerusalem, Jerusalem, Israel, 91904

Abstract: Previous studies demonstrated that retroviral proteins (including Tat, Rev and Rex) are localized to the nucleus. To our knowledge, nuclear localization of proteins translated from env or LTR regions of the Mouse Mammary Tumor Virus (MMTV) has not been reported. Monoclonal antibodies (MAbs) were raised against non-tumorigenic immunogenic S49 mouse lymphoma (T-25-Adh) variants. One MAb (M-66) recognized a 14 kD protein in nuclear extracts of both T-25-Adh and parental tumorigenic variants of S49 cells, but not in mouse splenocytes. M-66 identified 7 clones from a T-25-Adh cDNA expression library. All clones demonstrated extensive homology (more than 95%) to the env and LTR regions of MMTV. In parental, tumorigenic S49 variants, an additional unique, nuclear 21 kD protein was identified by M-66. This protein was immunoprecipitated by anti MMTV polyclonal antibodies. These findings suggest the localization of MMTV (env or LTR) proteins in the nucleus. The relevance of the above mentioned findings to the differing biological characteristics of the tumorigenic and non tumorigenic S49 cells is under investigation.
Keywords: Animal Antibodies, Monoclonal/DIAGNOSTIC USE Antigens, Viral/*METABOLISM Cell Nucleus/IMMUNOLOGY Gene Products, env/IMMUNOLOGY/METABOLISM Lymphoma/GENETICS/*IMMUNOLOGY Mammary Tumor Viruses, Mouse/*IMMUNOLOGY Mice Repetitive Sequences, Nucleic Acid ABSTRACTKWDanimalantibodies,monoclonal/diagnosticuseantigens,viral/KWDmetabolismcellnucleus/immunologygeneproducts,env/immunology/metabolismlymphoma/genetics/KWDimmunologymammarytumorviruses,mouse/KWDimmunologymicerepetitivesequences,nucleicacidabstract
980228
M9820765

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