Improved gene transfer into human cancer cell lines using retrovirus with the gibbon ape leukemia virus envelope and a cationic liposome (Meeting abstract).

Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.

Improved gene transfer into human cancer cell lines using retrovirus with the gibbon ape leukemia virus envelope and a cationic liposome (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 38:A1188 1997. Unique Identifier : AIDSLINE ICDB/98638188
Kim Y-S; Lim HK; Min JS; Institute for Cancer Research, College of Medicine, Yonsei; University, Seoul 120-752, Korea

Abstract: Efficient in vivo gene transfer is an important goal for cancer gene therapy. Although adenoviral vectors have proven effective for this purpose in animal models, the ability to achieve comparable gene transfer with retroviral vectors would provide potentially desirable safety and toxicity features for clinical studies. A potential strategy to improve gene transfer efficiency is the utilization of alternative retroviral envelopes. Therefore, we compared the transduction efficiency of human cancer cells with retroviruses bearing the envelope of amphotropic murine leukemia virus (MuLV) and gibbon ape leukemia virus (GALV). Transduction efficiency of the human cancer cell lines with retroviruses bearing GALV envelope was 5- to 30-fold higher than that with retroviruses bearing MuLV envelope. We also tested cationic lipids for enhancement of transduction efficiency. In the presence of polybrene, 30% of the cells were transduced by the retroviruses bearing GALV envelope at a multiplicity of infection (MOI) of 10 as demonstrated by X-Gal staining. However, 80% of the human cancer cells were transduced in the presence of a cationic liposome at same MOI. These results suggest a new, more effective strategy of gene therapy for malignant disease using combination of a cationic liposome and GALV envelope-bearing retrovirus-mediated gene transfer.

Keywords: Gene Therapy *Gene Transfer Human Leukemia Virus, Gibbon Ape/*GENETICS Liposomes Transduction, Genetic Tumor Cells, Cultured ABSTRACT
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