The V1/V2 region of human immunodeficiency virus type 1 modulates the sensitivity to neutralization by soluble CD4 and cellular tropism. NLM AIDSLINE Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.

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The V1/V2 region of human immunodeficiency virus type 1 modulates the sensitivity to neutralization by soluble CD4 and cellular tropism.

AIDS Res Hum Retroviruses. 1997 Oct 10;13(15):1291-9. Unique Identifier : AIDSLINE MED/97479765
Morikita T; Maeda Y; Fujii S; Matsushita S; Obaru K; Takatsuki K; The Second Department of Internal Medicine, Kumamoto University; School of Medicine, Japan.

Abstract: A primary isolate (KMT) of human immunodeficiency virus type 1 (HIV-1) resistant to recombinant soluble CD4 (rsCD4) was isolated from an HIV-1-infected individual and grown in a T lymphoid cell line. KMT isolate passaged on CEM cells (KMT/CEM) was still resistant to rsCD4. The V1/V2 and V3 regions of the viral envelope glycoprotein are thought to be involved in various biological phenotypes. To determine the exact envelope region of the KMT isolate responsible for sensitivity to rsCD4 and cellular tropism, we performed sequence analysis of KMT and KMT/CEM isolates. Sequence analysis of the KMT isolate showed that the sequence of the V3 region was relatively homogeneous, whereas a considerable heterogeneity of the V1/V2 region was noted. In contrast, the sequences of the V1 to V3 regions were homogeneous in KMT/CEM isolates. Analysis of NL4-3-based recombinant viruses with amplified sequences of the V1 to V3 regions from KMT and KMT/CEM isolates showed that the V1/V2 region modulated the sensitivity to rsCD4. A change in resistance to rsCD4 by the V1/V2 region was associated with the ability of the isolate to replicate in macrophages and efficiently replicate in T lymphoid cell lines. A change to an isolate sensitive to rsCD4 was associated with reduced replication efficiency in T lymphoid cell lines. Our results suggest that the V1/V2 region is involved in modulating the sensitivity to rsCD4, macrophage tropism, and replication efficiency in T lymphoid cell lines.
Keywords: Amino Acid Sequence Animal Antigens, CD4/GENETICS/*IMMUNOLOGY Cells, Cultured Chimera/GENETICS/IMMUNOLOGY Chimeric Proteins/GENETICS/IMMUNOLOGY Cloning, Molecular CD4-Positive T-Lymphocytes/IMMUNOLOGY/VIROLOGY COS Cells HIV Antigens/GENETICS/IMMUNOLOGY HIV Envelope Protein gp120/*GENETICS/IMMUNOLOGY HIV Infections/*GENETICS/*IMMUNOLOGY HIV-1/GROWTH & DEVELOPMENT/*GENETICS/*IMMUNOLOGY Macrophages/VIROLOGY Molecular Sequence Data Neutralization Tests Peptide Fragments/*GENETICS/IMMUNOLOGY Polymerase Chain Reaction Recombinant Proteins/IMMUNOLOGY Recombination, Genetic Sequence Alignment Sequence Analysis, DNA Support, Non-U.S. Gov't Tropism/GENETICS/IMMUNOLOGY JOURNAL ARTICLEKWDaminoacidsequenceanimalantigens,cd4/genetics/KWDimmunologycells,culturedchimera/genetics/immunologychimericproteins/genetics/immunologycloning,molecularcd4-positivet-lymphocytes/immunology/virologycoscellshivantigens/genetics/immunologyhivenvelopeproteingp120/KWDgenetics/immunologyhivinfections/KWDgenetics/KWDimmunologyhiv-1/growth&development/KWDgenetics/KWDimmunologymacrophages/virologymolecularsequencedataneutralizationtestspeptidefragments/KWDgenetics/immunologypolymerasechainreactionrecombinantproteins/immunologyrecombination,geneticsequencealignmentsequenceanalysis,dnasupport,non-uKWDsKWDgov'ttropism/genetics/immunologyjournalarticle
980228
M9820724

Copyright © 1998 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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