Inhibition of FIV replication by a ribozyme that targets the Rev response element. NLM AIDSLINE Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.

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Inhibition of FIV replication by a ribozyme that targets the Rev response element.

AIDS Res Hum Retroviruses. 1997 Oct 10;13(15):1333-9. Unique Identifier : AIDSLINE MED/97479769
Mergia A; Blackwell J; Chari S; Department of Pathobiology, College of Veterinary Medicine,; University of Florida, Gainesville 32610-0145, USA.

Abstract: The feline immunodeficiency virus (FIV) Rev protein and its cognate sequence the Rev response element (RRE) are essential for virus replication. Thus, the inhibition of either Rev or RRE function can significantly inhibit FIV replication. In the present study, we constructed a ribozyme that targets the RRE sequence and determined its ability to inhibit FIV replication. The RRE ribozyme cleaved the target molecule both in vitro and in FIV-infected cells. Furthermore, FIV replication was reduced significantly in the presence of the RRE ribozyme. FIV provides a good animal model system with which to develop novel antiviral strategies for the human immunodeficiency virus.
Keywords: Feline Acquired Immunodeficiency Syndrome/*VIROLOGY Genetic Vectors Genome, Viral Immunodeficiency Virus, Feline/*GROWTH & DEVELOPMENT Nuclear Proteins/*ANTAGONISTS & INHIB Polymerase Chain Reaction RNA-Directed DNA Polymerase/METABOLISM RNA, Catalytic/*CHEMICAL SYNTHESIS/METABOLISM/*PHARMACOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Tumor Cells, Cultured Virus Replication/*DRUG EFFECTS JOURNAL ARTICLEKWDfelineacquiredimmunodeficiencysyndrome/KWDvirologygeneticvectorsgenome,viralimmunodeficiencyvirus,feline/KWDgrowth&developmentnuclearproteins/KWDantagonists&inhibpolymerasechainreactionrna-directeddnapolymerase/metabolismrna,catalytic/KWDchemicalsynthesis/metabolism/KWDpharmacologysupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDtumorcells,culturedvirusreplication/KWDdrugeffectsjournalarticle
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