Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.
Characterization of lymphocytes in the adult rat testis by flow cytometry: effects of activin and transforming growth factor beta on lymphocyte subsets in vitro.
Biol Reprod. 1998 Apr;58(4):943-51. Unique Identifier : AIDSLINE MED/98206801 Tompkins AB; Hutchinson P; de Kretser DM; Hedger MP; Institute of Reproduction and Development, Monash University, Clayton,; Victoria, Australia.
Abstract:
The rat testis is considered to be an immunologically privileged site because of its reduced capacity to support antigen-specific immune responses. To understand this phenomenon, it is essential to characterize both the lymphocyte subpopulations normally present in the testis and their regulation by testicular cytokines. Peripheral blood was obtained from adult male Dark Agouti or Sprague-Dawley rats, and testicular interstitial tissue was collected after perfusion of the testes to remove blood. Blood and testis lymphocytes were isolated using discontinuous Percoll density gradients, and the testicular lymphocytes were further purified by selective adherence to remove mononuclear phagocytes. The isolated lymphocytes were analyzed by flow cytometry using specific monoclonal antibodies and fluorescein labeling and were enumerated as total T cells, CD4+ T cells, CD8+ T cells, B cells, and natural killer (NK) cells. In contrast to peripheral blood, in which the CD4+ T-cell subset was the major lymphocyte subset, rat testis T cells were predominantly of the CD8+ subset, and a large population of NK cells also were present. Subsequently, peripheral blood lymphocytes were stimulated with the polyclonal T-cell activator, phytohemagglutinin, and cultured in the presence of activin, inhibin, or transforming growth factor beta (TGFbeta) prior to flow cytometric analysis. Activin and TGFbeta suppressed T-cell proliferation without any selective effect on either T-cell subset, and inhibin had no effect. The predominance of CD8+ T cells and NK cells, and the relatively minor proportion of CD4+ T cells, are consistent with both increased cellular immune surveillance and a reduced capacity for initiating antigen-specific immune responses in the adult rat testis.
Keywords: *Flow Cytometry *Inhibin/PHARMACOLOGY *Lymphocytes/IMMUNOLOGY *Testis/CYTOLOGY *Testis/IMMUNOLOGY *Transforming Growth Factor beta/PHARMACOLOGY 980830
M9881180
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Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.
