Surface expression of TRAIL/Apo-2 ligand in activated mouse T and B cells. NLM AIDSLINE Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.

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Surface expression of TRAIL/Apo-2 ligand in activated mouse T and B cells.

Eur J Immunol. 1998 May;28(5):1492-8. Unique Identifier : AIDSLINE MED/98264616
Mariani SM; Krammer PH; Division of Immunogenetics, German Cancer Research Center, Heidelberg.

Abstract: Like other members of the TNF family, TRAIL/Apo-2 ligand induces apoptosis in sensitive target cells in a caspase-dependent fashion. We recently found that TRAIL may be constitutively expressed on the surface of mouse and human tumor cells of T and B origin. To define the pattern of TRAIL expression in normal immune cells, freshly isolated splenocytes, Concanavalin A/IL-2-activated T cells and lipopolysaccharide-activated B cells were analyzed by surface staining with or without secondary stimulation. Activated, but not resting, CD3+ cells expressed TRAIL in an activation-dependent fashion. Conversely, freshly isolated B220+ cells displayed surface TRAIL and CD95L that were retained following activation. Restimulation with the protein kinase C activator phorbol 12-myristate 13-acetate and the calcium ionophore ionomycin or an agonistic anti-CD3 monoclonal antibody induced significant up-regulation of surface TRAIL and CD95L in CD3+, TCRalphabeta cells with CD4+ or CD8+ phenotype. Similarly to CD95L, TRAIL up-regulation was protein synthesis dependent and cyclosporin A sensitive. These results indicate that both TRAIL and CD95L are displayed on the cell surface of activated immune cells and may thus represent complementary effector pathways in the regulatory functions of T and B cells.
Keywords: *B-Lymphocyte Subsets/METABOLISM *Lymphocyte Transformation *Membrane Glycoproteins/BIOSYNTHESIS *T-Lymphocyte Subsets/METABOLISM *Tumor Necrosis Factor/BIOSYNTHESISKWDb-lymphocytesubsets/metabolismKWDlymphocytetransformationKWDmembraneglycoproteins/biosynthesisKWDt-lymphocytesubsets/metabolismKWDtumornecrosisfactor/biosynthesis
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Copyright © 1998 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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