The use of multiple antigenic peptide (MAP) in the immunodiagnosis of human immunodeficiency virus infection.

The use of multiple antigenic peptide (MAP) in the immunodiagnosis of human immunodeficiency virus infection.

Biochem Mol Biol Int. 1997 Nov;43(4):713-21. Unique Identifier : AIDSLINE MED/98046541
Shin SY; Lee MK; Kim SY; Jang SY; Hahm KS; Peptide Engineering Research Unit, Korea Research Institute of; Bioscience and Biotechnology, KIST, Taejon, Korea.

Abstract: In order to develop an ELISA system for the antibody detection of HIV-1 or HIV-2 infections, MAPs for HIV-1 gp41(584-618) and HIV-2 gp36(574-602) corresponding to the immunodominant regions of HIV-1 gp41 and HIV-2 gp36 were used as coating antigens in the ELISA. The MAPs were synthesized by the solid phase method using Fmoc-Lys(Fmoc)-OH and their molecular weights were confirmed by tricine gel electrophoresis. The MAPs reacted with all HIV positive sera (64 samples), but did not react with HIV negative sera (48 samples) obtained from healthy blood donors. The MAPs showed high sensitivity and specificity in anti-HIV 1/2 combo panel and anti-HIV-1 seroconversion panels. The results indicated that the ELISA system using synthetic MAPs of gp41(584-618) and gp36(574-602) as coating antigens can be used as an analytical system for the immunodiagnosis of HIV-1 or HIV-2 infections.

Keywords: *HIV Infections/DIAGNOSIS *HIV Infections/IMMUNOLOGY *Immunodominant Epitopes/DIAGNOSTIC USE *Peptide Fragments/IMMUNOLOGY
980430
M9841816

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