Cytomegalovirus monitoring by polymerase chain reaction of whole blood samples from patients undergoing autologous bone marrow or peripheral blood progenitor cell transplantation. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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Cytomegalovirus monitoring by polymerase chain reaction of whole blood samples from patients undergoing autologous bone marrow or peripheral blood progenitor cell transplantation.

J Infect Dis. 1997 Jun;175(6):1490-3. Unique Identifier : AIDSLINE MED/97323995
Hebart H; Schroder A; Loffler J; Klingebiel T; Martin H; Wassmann B; Gerneth F; Rabenau H; Jahn G; Kanz L; Muller CA; Einsele H; Medizinische Universitatsklinik, Abteilung II, Kinderklinik, Institut; fur Medizinische Informationsverarbeitung, and Abteilung fur; Medizinische Virologie, Universitat Tubingen, Germany.

Abstract: Sensitive screening for cytomegalovirus (CMV) by polymerase chain reaction (PCR) following autologous bone marrow or peripheral blood progenitor cell transplantation has not been evaluated. In a three-center study, 98 autograft transplant recipients were prospectively screened for CMV infection by PCR and culture techniques. At a median of 20 days (range, 3-28) after transplantation, 21 (39.6%) of 53 CMV-seronegative patients were PCR positive for CMV, and at a median of 17 days (range, 7-84) after transplantation, 19 (42.2%) of 45 CMV-seropositive patients were PCR positive for CMV. Low-level DNAemia (1-10 fg CMV DNA/mL blood) occurred for 1 week in 31 patients but was never associated with CMV disease. Of 9 patients who presented with at least two consecutive positive PCR results, 1 developed CMV pneumonia. No patients died because of CMV disease. Screening for CMV infection by PCR had a negative predictive value of 100% (as also observed after allogeneic transplantation), but its positive predictive value was significantly lower.
Keywords: *Blood Transfusion, Autologous *Bone Marrow Transplantation *Cytomegalovirus/ISOLATION & PURIF *Cytomegalovirus Infections/VIROLOGY *DNA, Viral/BLOOD *Polymerase Chain Reaction/METHODSKWDbloodtransfusion,autologousKWDbonemarrowtransplantationKWDcytomegalovirus/isolation&purifKWDcytomegalovirusinfections/virologyKWDdna,viral/bloodKWDpolymerasechainreaction/methods
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Copyright © 1997 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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