A subpopulation of immature blood dendritic cells is highly susceptible to infection by macrophage-tropic HIV-1. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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A subpopulation of immature blood dendritic cells is highly susceptible to infection by macrophage-tropic HIV-1.

Conf Adv AIDS Vaccine Dev. 1997 May 4-7;:18. Unique Identifier : AIDSLINE MED/97927010
Crawford K; Alper C; Shi B; Vasir D; Gabuzda D; Dana Farber Cancer Institute, Boston, MA. Fax: (617) 632-3113.

Abstract: Dendritic cells (DC) are unique antigen-presenting cells that are widely distributed in lymphoid and non-lymphoid tissues. Previous studies have suggested that DC play an important role in the transmission of HIV-1 to T cells. However, controversy exists regarding whether DC are directly susceptible to HIV-1 infection. We used a new isolation method to purify a CD2+CD14+ DC population from peripheral blood. When allowed to mature in culture, this population expresses the DC marker CD83 (HB-15) and exhibits morphological and functional features characteristic of DC. Freshly isolated CD2+CD14+ blood DC were highly infectable with the macrophage-tropic BaL HIV-1 isolate, but not with a T cell line-tropic isolate. HIV-1 infection was cytopathic, causing DC apoptosis, syncytia formation, and cell lysis. Virion production occurred primarily within large cytoplasmic vacuoles, rather than by budding at the cell surface. These studies demonstrate that a subpopulation of immature blood DC is highly susceptible to infection with macrophage-tropic HIV-1 and may be an important target for primary HIV-1 infection in vivo.
Keywords: *Dendritic Cells/VIROLOGY *HIV-1/PHYSIOLOGY *Macrophages/VIROLOGY *TropismKWDdendriticcells/virologyKWDhiv-1/physiologyKWDmacrophages/virologyKWDtropism
971130
M97B1206

Copyright © 1997 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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