Infection of cats with FIV by injection with cloned proviral DNA. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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Infection of cats with FIV by injection with cloned proviral DNA.

Conf Adv AIDS Vaccine Dev. 1997 May 4-7;:23. Unique Identifier : AIDSLINE MED/97927015
Sparger EE; Louie H; Ziomeck AM; Luciw PA; Department of Veterinary Medicine and Epidemiology, University of; California, Davis, CA. Fax: (916) 752-0414.

Abstract: Establishment of infection of animals with a viral clone is important for investigating viral determinants of pathogenesis and monitoring sequence changes in the viral genome in vivo, and may find utility as a means of immunization with live-attenuated virus. To test the efficiency of intramuscular (IM) injection of cloned proviral plasmid DNA for establishing feline immunodeficiency virus (FIV) infection in specific pathogen free SPF) cats, groups (n=3) of cats were inoculated IM with either 300 micrograms, 100 micrograms, or 30 micrograms of plasmid DNA containing the infectious molecular clone, FIV-pPPR. A fourth group of cats (n=3) was inoculated intradermally (ID) with 30 micrograms of FIV-pPPR plasmid DNA. For comparison, a fifth group n=5) received 10(3) TCID(50) of a live virus stock of FIV-pPPR by intraperitoneal (IP) inoculation. Inoculation by IM injection with 100 to 300 micrograms of infectious FIV-pPPR proviral produced infection detectable by both anti-viral antibody and virus isolation from peripheral blood mononuclear cells (PBMC). Inoculation by IM injection with 30 micrograms of proviral DNA resulted in infection in 2 of 3 inoculated cats. ID injection with 30 micrograms of proviral DNA induced infection in 1 of 3 cats. Induction of anti-viral antibody and viremia was delayed in cats inoculated with 30 micrograms when compared to cats inoculated with either 100 micrograms or 300 micrograms of proviral DNA. This study indicates that 100 micrograms of FIV proviral DNA may replace infectious virion preparations as inocula for pathogenesis and immunization studies.
Keywords: *DNA, Viral/ADMINISTRATION & DOSAGE *Feline Acquired Immunodeficiency Syndrome/TRANSMISSION *Immunodeficiency Virus, Feline/GENETICS *Proviruses/GENETICSKWDdna,viral/administration&dosageKWDfelineacquiredimmunodeficiencysyndrome/transmissionKWDimmunodeficiencyvirus,feline/geneticsKWDproviruses/genetics
971130
M97B1203

Copyright © 1997 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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