HIV-1 inhibitory activity of CD8+ cell supernatants is distinct from that of RANTES, MIP-1alpha and MIP-1beta. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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HIV-1 inhibitory activity of CD8+ cell supernatants is distinct from that of RANTES, MIP-1alpha and MIP-1beta.

Conf Adv AIDS Vaccine Dev. 1997 May 4-7;:40. Unique Identifier : AIDSLINE MED/97927026
Klotman ME; Mosoian A; Teixeira A; Sperber K; Piwoz J; Caron E; Mt. Sinai School of Medicine, New York, NY. Fax: (212) 534-3240.

Abstract: CD8+ supernatant (sups)inhibition of HIV-1 replication in lymphocytes is at least partially due to the chemokines (ccs) RANTES, MIP-1alpha and MIP-1beta. To further define the inhibitory activity of CD8+ sups we compared the inhibitory activity of transformed CD8+ cells with that of recombinant chemokines. Sups from Herpesvirus Saimiri (HVS) strain C-488-immortalized CD8+ cells were screened for HIV-1 inhibitory activity in CD4+ transformed and primary cells and in primary macrophages utilizing both primary and laboratory-adapted strains of HIV-1 and compared to the inhibitory activity of a mixture of RANTES, MIP-1alpha and MIP-1beta, each at a concentration of 200 ng/ml. Sups from CD8+ cells from infected as well as a normal donor had potent inhibitory activity against HIV-1 Ba-l, IIIB, MN and primary isolates in transformed as well as in primary CD4+ cells. Furthermore, sups potently inhibited Ba-l in primary macrophages. The chemokine cocktail at 200 ng/ml had no activity against IIIB in lymphocytes and failed to inhibit Ba-l in the majority of primary macrophage cultures. Increasing the dose to 400 ng/ml had modest inhibitory effects. Concentration of the CD8+ sup and further purification by size fractionation, ion exchange chromatography followed by reverse-phase chromatography of the active peak revealed the presence of a single band on SDS gel that still retained full inhibitory activity and was negative by Western blot for RANTES, MIP-1alpha and MIP-1beta. Thus there are additional distinct inhibitory factors in CD8+ cells and transformed cells allow purification of this activity.
Keywords: *Chemokines/IMMUNOLOGY *CD8-Positive T-Lymphocytes/IMMUNOLOGY *Virus Inhibitors/PHYSIOLOGYKWDchemokines/immunologyKWDcd8-positivet-lymphocytes/immunologyKWDvirusinhibitors/physiology
971130
M97B1195

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