Distribution of HIV-1 subtypes seen in an AIDS clinic in Sao Paulo City, Brazil. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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Distribution of HIV-1 subtypes seen in an AIDS clinic in Sao Paulo City, Brazil.

AIDS. 1996 Nov;10(13):1579-84. Unique Identifier : AIDSLINE MED/97085708
Sabino EC; Diaz RS; Brigido LF; Learn GH; Mullins JI; Reingold AL; Duarte AJ; Mayer A; Busch MP; Instituto Adolfo Lutz, Sao Paulo, Brazil.

Abstract: OBJECTIVE: To determine the distribution of HIV-1 subtypes in Sao Paulo, Brazil. METHODS: Samples were obtained from 80 consecutive HIV-1-infected individuals attending the Immunodeficiency Clinic at the University of Sao Paulo in 1993. Peripheral blood mononuclear cells (PBMC) were separated by Ficoll-Hypaque gradient and a portion was used for routine CD4 counts; the remainder were frozen. PBMC were proteinase-K-digested and DNA-purified by organic extraction. Samples were amplified for the env region of HIV, and envelope sequence subtypes determined by heteroduplex mobility analysis using prototypic subtypes as references. A subset of these were also sequenced through the C2-V3 region of env. RESULTS: A total 69 of 80 samples yielded env polymerase chain reaction product enabling subtype determination; samples that did not amplify were those with low DNA yields. Among 12 injecting drug users (IDU) or sexual partners of IDU, four were typed as clade F and eight as clade B. Forty-three homosexual men or female sexual partners of bisexual men were typed as clade B. The 14 additional cases without known risk factors were typed as clade B. CONCLUSION: These data suggest that subtype F is related to injecting drug use in Brazil.
Keywords: *Acquired Immunodeficiency Syndrome/VIROLOGY *Genes, env *HIV-1/CLASSIFICATION *HIV-1/GENETICSKWDacquiredimmunodeficiencysyndrome/virologyKWDgenes,envKWDhiv-1/classificationKWDhiv-1/genetics
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