V3 loop sequence analysis of seven HIV type 1 group O isolates phenotyped in peripheral blood mononuclear cells and MT-2 cells.

Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

V3 loop sequence analysis of seven HIV type 1 group O isolates phenotyped in peripheral blood mononuclear cells and MT-2 cells.

AIDS Res Hum Retroviruses. 1996 Nov 1;12(16):1503-7. Unique Identifier : AIDSLINE MED/97068247
De Jong J; Simon F; Van der Groen G; Baan E; Saragosti S; Brun-Vezinet F; Goudsmit J; Department of Human Retrovirology, Academic Medical Centre, Amsterdam,; The Netherlands.

Abstract: HIV-1-infected individuals from which syncytium-inducing (SI) viruses are isolated most often progress more rapidly to AIDS than individuals carrying only non-syncytium-inducing (NSI) viruses. The syncytium-inducing capacity of virus isolates is commonly determined in conjunction to replication in MT-2 cells. Comparison of HIV-1 env sequences and a site-directed mutagenesis study have indicated that the presence of a positively charged amino acid at position 11 or 25 in the V3 loop is minimally required for the SI capacity of HIV-1 subtype B viruses. Studies have also shown a similar correlation between positively charged signature amino acids in the V3 loop and syncytium formation in MT-2 cells for HIV-1 subtypes A, D, and E. In the present study virus phenotype was determined and compared to the V3 loop sequence of seven HIV-1 group O isolates. Three of the HIV-1 group O isolates showed the NSI/non-MT-2 tropic phenotype and two showed the SI/MT-2 tropic phenotype, whereas two isolates presented an uncommon NSI/MT-2 tropic phenotype. The V3 loop of the two SI/MT-2 tropic isolates had a high net positive charge and contained a positively charged amino acid at position 11 or 25. The V3 loop of the two NSI/MT-2 tropic isolates had a low net positive charge and contained a single positively charged amino acid at position 37.

Keywords: *HIV Envelope Protein gp120/GENETICS *HIV-1/CLASSIFICATION *Leukocytes, Mononuclear/VIROLOGY *Peptide Fragments/GENETICS *T-Lymphocytes/VIROLOGY
970530
M9751924

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