Activation of the HIV type 1 long terminal repeat by X-irradiation involves two main Re1/NF-kappa B DNA-binding complexes.

Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

Activation of the HIV type 1 long terminal repeat by X-irradiation involves two main Re1/NF-kappa B DNA-binding complexes.

AIDS Res Hum Retroviruses. 1996 Nov 1;12(16):1519-27. Unique Identifier : AIDSLINE MED/97068249
Faure E; Lecine P; Imbert J; Champion S; Institut de Chimie Biologique, Universite de Provence, Marscelle,; France.

Abstract: Transcription of human immunodeficiency virus type 1 (HIV-1) is regulated by multiple cis-acting regulatory elements located in the viral long terminal repeats (LTRs). HIV-1 LTR enhancer is activated by a variety of heterologous viral, chemical, and physical agents. Studies have demonstrated that irradiation by X-rays induces transcription under the control of the HIV-1 LTR and that ionizing radiations activate DNA binding of the nuclear transcription factor NF-kappa B. Using various constructs expressing a reporter gene under the control of complete or deleted LTRs of HIV-1, we evidenced that a sequence located in the U3 region was involved in X-ray activation of the HIV-1 LTR in the human colonic carcinoma cell line HT29. The cis-acting element conferring X-ray responsiveness is indistinguishable from HIV NF-kappa B tandem repeat binding sites (HIV-1, kappa B). The present work has examined the effects of X-irradiation on the NF-kappa B transcription factor. Furthermore, we characterized the subunit composition of the two major nuclear NF-kappa B complexes that bind HIV-1 kappa B after X-ray irradiation.

Keywords: *HIV Long Terminal Repeat/RADIATION EFFECTS *HIV-1/RADIATION EFFECTS *NF-kappa B/RADIATION EFFECTS
970530
M9751922

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