Distribution of human herpesvirus-8 sequences throughout the spectrum of AIDS-related neoplasia. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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Distribution of human herpesvirus-8 sequences throughout the spectrum of AIDS-related neoplasia.

AIDS. 1996 Aug;10(9):941-9. Unique Identifier : AIDSLINE MED/97006433
Gaidano G; Pastore C; Gloghini A; Cusini M; Nomdedeu J; Volpe G; Capello D; Vaccher E; Bordes R; Tirelli U; Saglio G; Carbone A; Department of Medical Science, University of Turin, Italy.

Abstract: OBJECTIVE: AIDS frequently associates with certain malignancies, including Kaposi's sarcoma, non-Hodgkin's lymphoma (NHL), and anogenital neoplasia. In this study we aimed to define the frequency of infection by human herpesvirus (HHV)-8 throughout the spectrum of AIDS-related neoplasia in Europe. DESIGN: A tumour panel representative of the distinct types of AIDS-related neoplasms was tested for the presence of HHV-8 DNA sequences. Autologous uninvolved tissues were also tested in selected cases. METHODS: The presence of HHV-8 DNA sequences was assayed by a combination of polymerase chain reaction followed by oligohybridization and Southern blot hybridization of genomic DNA with an HHV-8-specific probe. RESULTS: HHV-8 sequences were detected in 100% of AIDS-related Kaposi's sarcoma (all 35 cases). Among AIDS-related NHL, HHV-8 sequences selectively clustered with body-cavity-based lymphomas (BCBL; all three cases), although they were consistently negative in small non-cleaved cell lymphomas (none in 18 cases), diffuse large cell lymphomas (none in seven), or anaplastic large cell lymphomas (none in three). No HHV-8 sequences were found in cases of anogenital neoplasia (out of 14) or Hodgkin's disease (out of three). HHV-8 DNA sequences were also positive in the uninvolved skin of all six AIDS-related Kaposi's sarcoma patients, but not in the circulating lymphocytes of a BCBL patient. Positivity for HHV-8 sequences occurred in patients belonging to all major AIDS risk categories. CONCLUSIONS: These data confirm that HHV-8 sequences associate at high frequency with selected types of AIDS-related neoplasia, namely Kaposi's sarcoma and BCBL, although they are consistently absent in other types of AIDS-NHL and AIDS-related anogenital neoplasia.
Keywords: Acquired Immunodeficiency Syndrome/*COMPLICATIONS Anus Neoplasms/ETIOLOGY/*VIROLOGY AIDS-Related Opportunistic Infections/ETIOLOGY/*GENETICS/VIROLOGY Cervical Intraepithelial Neoplasia/ETIOLOGY/*VIROLOGY DNA Primers DNA, Viral/*ANALYSIS Female Herpesviridae/*GENETICS/ISOLATION & PURIF Herpesviridae Infections/ETIOLOGY/GENETICS/VIROLOGY Human Lymphoma, Non-Hodgkin's/ETIOLOGY/*VIROLOGY Male Polymerase Chain Reaction Sarcoma, Kaposi's/ETIOLOGY/*VIROLOGY Support, Non-U.S. Gov't Tumor Virus Infections/ETIOLOGY/GENETICS/VIROLOGY JOURNAL ARTICLEKWDacquiredimmunodeficiencysyndrome/KWDcomplicationsanusneoplasms/etiology/KWDvirologyaids-relatedopportunisticinfections/etiology/KWDgenetics/virologycervicalintraepithelialneoplasia/etiology/KWDvirologydnaprimersdna,viral/KWDanalysisfemaleherpesviridae/KWDgenetics/isolation&purifherpesviridaeinfections/etiology/genetics/virologyhumanlymphoma,non-hodgkin's/etiology/KWDvirologymalepolymerasechainreactionsarcoma,kaposi's/etiology/KWDvirologysupport,non-uKWDsKWDgov'ttumorvirusinfections/etiology/genetics/virologyjournalarticle
970330
M9731506

Copyright © 1997 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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