Induction of somatic mutation in a human B cell line in vitro.

Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

Induction of somatic mutation in a human B cell line in vitro.

Immunity. 1997 Jan;6(1):35-46. Unique Identifier : AIDSLINE MED/97205331
Denepoux S; Razanajaona D; Blanchard D; Meffre G; Capra JD; Banchereau J; Lebecque S; Schering-Plough Laboratory for Immunological Research, Dardilly,; France.

Abstract: Both the B cell-surface trigger(s) and the intracellular molecular mechanism(s) of somatic hypermutation in immunoglobulin Ig) variable region genes remain unknown, partly because of the lack of a simple and reproducible in vitro model. Here, we show that upon surface immunoglobulin cross-linking followed by co-culture with activated cloned T cells, the Burkitt's lymphoma cell line BL2 is induced to mutate its IgV(H) gene. Repeated activation of BL2 cells increased the frequency of mutation. The in vitro-induced mutations, which do not affect the IgM constant region, are point mutations distributed over the entire V(H)DJ(H) gene segment and do not show evidence of antigen-driven selection.

Keywords: *Antibody Affinity *B-Lymphocytes/CYTOLOGY *Genes, Immunoglobulin/GENETICS *Immunoglobulin Variable Region/GENETICS *Immunoglobulins, mu-Chain/GENETICS *Lymphocyte Transformation *Mutation *Receptors, Antigen, B-Cell/PHYSIOLOGY
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