The HIV-1 Nef protein acts as a connector with sorting pathways in the Golgi and at the plasma membrane. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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The HIV-1 Nef protein acts as a connector with sorting pathways in the Golgi and at the plasma membrane.

Immunity. 1997 Jan;6(1):67-77. Unique Identifier : AIDSLINE MED/97205334
Mangasarian A; Foti M; Aiken C; Chin D; Carpentier JL; Trono D; Infectious Disease Laboratory, The Salk Institute for Biological; Studies, La Jolla, California 92037, USA.

Abstract: The HIV Nef protein down-regulates the cell surface expression of CD4 and of MHC I at least in part through accelerated endocytosis. To investigate further the mechanism of this effect, we created chimeric integral membrane proteins comprising the extracellular and transmembrane regions of CD4 or CD8 and Nef as the cytoplasmic domain. These fusion molecules could down-modulate CD4 in trans in a dileucine-dependent manner. Furthermore, in spite of lacking receptor-derived internalization signals, the Nef-containing chimeras underwent both Golgi retention and rapid endocytosis via clathrin-coated pits. Taken together, these data suggest that Nef down-regulates CD4 and probably MHC I by physically connecting these receptors with sorting pathways in the Golgi and at the plasma membrane.
Keywords: *Antigens, CD4/METABOLISM *Antigens, CD8/METABOLISM *Cell Membrane/METABOLISM *Gene Products, nef/METABOLISM *Golgi Apparatus/METABOLISM *HIV-1/METABOLISMKWDantigens,cd4/metabolismKWDantigens,cd8/metabolismKWDcellmembrane/metabolismKWDgeneproducts,nef/metabolismKWDgolgiapparatus/metabolismKWDhiv-1/metabolism
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M9761190

Copyright © 1997 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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