HIV-1 integrase pharmacophore: discovery of inhibitors through three-dimensional database searching. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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HIV-1 integrase pharmacophore: discovery of inhibitors through three-dimensional database searching.

J Med Chem. 1997 Mar 14;40(6):920-9. Unique Identifier : AIDSLINE MED/97236952
Nicklaus MC; Neamati N; Hong H; Mazumder A; Sunder S; Chen J; Milne GW; Pommier Y; Laboratory of Medicinal Chemistry, National Cancer Institute, National; Institutes of Health, Bethesda, Maryland 20892-4255, USA.

Abstract: Starting from a known inhibitor of human immunodeficiency virus type 1 (HIV-1) integrase (IN); caffeic acid phenethyl ester CAPE), a putative three-point pharmacophore for binding of inhibitors to IN was derived. This pharmacophore was used to search the National Cancer Institute three-dimensional (3D) structural database. Out of the open, nonproprietary part of this database, comprising approximately 200000 compounds, 267 structures were found to match the pharmacophore in at least one conformation, and 60 of those were tested in an in vitro assay against HIV-1 IN. Out of these, 19 were found to inhibit both the 3'-processing and strand transfer of IN at micromolar concentrations. In order to test the validity of this pharmacophore, a small 3D database of 152 published IN inhibitors was built. A search in this database yielded a statistically significant correlation of the presence of this pharmacophore and the potency of the compounds. An automated pharmacophore identification procedure performed on this set of compounds provided additional support for the importance of this pharmacophore for binding of inhibitors to IN and hinted at a possible second pharmacophore. The role of aromatic moieties in the binding of ligands to HIV-1 IN through interactions with divalent metal cations, which are known to be necessary for activity of the enzyme, was explored in ab initio calculations.
Keywords: *Databases, Factual *HIV Integrase/METABOLISM *HIV Integrase Inhibitors/CHEMISTRY *HIV-1/ENZYMOLOGYKWDdatabases,factualKWDhivintegrase/metabolismKWDhivintegraseinhibitors/chemistryKWDhiv-1/enzymology
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M9772047

Copyright © 1997 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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