The use of SCID mice in biotechnology and as a model for human disease. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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The use of SCID mice in biotechnology and as a model for human disease.

Crit Rev Biotechnol. 1996;16(1):95-118. Unique Identifier : AIDSLINE MED/97090002
Sandhu JS; Boynton E; Gorczynski R; Hozumi N; Department of Surgery, University of Toronto, Ontario, Canada.

Abstract: The use of SCID (severe combined immunodeficient) mice in medical research and biotechnology has increased tremendously in recent years. This review outlines the major characteristics of these animals and the impediments that they pose to the engraftment of human cells and tissues. The development of our SCID mice pretreatment protocol (anti-asialo GM1 antisera and radiation) is described, and the results of xenotransplantation studies of human cells and tissues in these pretreated animals are outlined. Wherever possible, data from transplantation studies (of human tissues and cells) in pretreated and nonpretreated animals are compared. The potential of our pretreated SCID mice for medical research and biotechnology is discussed.
Keywords: Animal Antibodies, Monoclonal/BIOSYNTHESIS *Biotechnology Disease Models, Animal Gene Therapy Graft vs Host Disease/IMMUNOLOGY Human HIV Infections/THERAPY Lymphocyte Transfusion Mice *Mice, SCID Severe Combined Immunodeficiency/IMMUNOLOGY Support, Non-U.S. Gov't Transplantation Immunology Transplantation, Heterologous Vaccines, Synthetic/ISOLATION & PURIF JOURNAL ARTICLE REVIEW REVIEW, TUTORIALKWDanimalantibodies,monoclonal/biosynthesisKWDbiotechnologydiseasemodels,animalgenetherapygraftvshostdisease/immunologyhumanhivinfections/therapylymphocytetransfusionmiceKWDmice,scidseverecombinedimmunodeficiency/immunologysupport,non-uKWDsKWDgov'ttransplantationimmunologytransplantation,heterologousvaccines,synthetic/isolation&purifjournalarticlereviewreview,tutorial
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Copyright © 1997 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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