Comparison of the Vbeta repertoire in peripheral blood and in lymph nodes of HIV-infected subjects reveals skewed usage predominantly in CD8+ T cells.

Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

Comparison of the Vbeta repertoire in peripheral blood and in lymph nodes of HIV-infected subjects reveals skewed usage predominantly in CD8+ T cells.

Clin Immunol Immunopathol. 1996 Nov;81(2):200-9. Unique Identifier : AIDSLINE MED/97064133
Carbonari M; Cibati M; Pesce AM; Dell'Anna L; D'Offizi G; Angelici A; Uccini S; Fiorilli M; Department of Clinical Medicine, University of Rome La Sapienza,; Italy.

Abstract: Perturbations of the repertoire of variable-beta (Vbeta) regions of the T cell receptor have been observed in patients infected by HIV and have been attributed to stimulation by viral antigens or superantigens. We further sought for traces of HIV-induced perturbations by comparing Vbeta repertoire in peripheral blood and in lymphoid tissues of six infected patients. Vbeta expression was studied with a panel of 17 anti-Vbeta antibodies covering about 50% of the entire repertoire. We observed major divergences between lymph nodes and peripheral blood in the expression of several Vbeta segments, and these differences were significantly more frequent in CD8+ than in CD4+ T cells (P = 0.0097). Vbeta2 was perturbed in CD8 cells from all but one patient. One HIV-negative subject with localized reactive lymphadenopathy of unknown etiology had four perturbed Vbeta segments, including Vbeta2, in CD8+ cells, while another uninfected subject with an unreactive lymph node architecture had no perturbations. Our findings suggest that stimulation by HIV or by other antigens determines divergences in the Vbeta repertoire between lymphoid tissues and peripheral blood predominantly in CD8+ T cells.

Keywords: Adult Comparative Study CD4-Positive T-Lymphocytes/CHEMISTRY CD8-Positive T-Lymphocytes/*CHEMISTRY Female Flow Cytometry Human HIV Infections/BLOOD/*METABOLISM Immunoglobulin Variable Region/BLOOD Immunohistochemistry Lymph Nodes/*CHEMISTRY Lymphocyte Transformation Male Receptors, Antigen, T-Cell, alpha-beta/*BLOOD Support, Non-U.S. Gov't T-Lymphocytes/IMMUNOLOGY JOURNAL ARTICLE
970228
M9721894

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