Abnormalities of CD45 isoform expression in HIV infection. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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Abnormalities of CD45 isoform expression in HIV infection.

Clin Immunol Immunopathol. 1996 Nov;81(2):210-4. Unique Identifier : AIDSLINE MED/97064134
Mahalingam M; Pozniak A; McManus TJ; Senaldi G; Vergani D; Peakman M; Department of Immunology, King's College Hospital, London, United; Kingdom.

Abstract: Using monoclonal antibodies against CD45, CD45RA, CD45RB, and CD45RO, the surface density of expression and proportions of different CD45 isoforms were analyzed on CD4+ and CD8+ T lymphocyte subsets in HIV+ patients and controls. Analysis of the relative specific fluorescence (RSF) of CD45 isoforms showed a significant reduction in expression of total CD45 and its isoforms, CD45RA, CD45RO, and CD45RB, on CD4+ and CD8+ lymphocyte subsets in HIV+ patients compared with controls. However, expression of phosphotyrosine in the cytoplasm of CD4+ and CD8+ lymphocytes did not differ significantly between HIV+ patients and controls. Percentages of "naive" CD45RA+RO- CD4+ and CD8+ lymphocytes were lower than control values in all HIV+ patients (P < 0.001). Compared with controls, HIV+ patients had similar percentages of "memory" CD45RA-RO+ CD4+ lymphocytes but significantly higher percentages of memory CD8+ lymphocytes (P < 0.001). Percentages of memory CD8+ lymphocytes were lowest in patients with AIDS (P < 0.001). The consistent reduction in surface expression of all CD45 isoforms observed in our study may be of relevance to the impaired T cell activation characteristic of HIV infection. Also, our results argue against the selective depletion of memory CD4+ lymphocytes in HIV-induced disease.
Keywords: Adult Antigens, CD45/IMMUNOLOGY Cytoplasm/CHEMISTRY CD4-Positive T-Lymphocytes/IMMUNOLOGY CD8-Positive T-Lymphocytes/IMMUNOLOGY Female Fluorescent Antibody Technique Human HIV Infections/*GENETICS/*IMMUNOLOGY Lymphocytes/ULTRASTRUCTURE Male Phosphotyrosine/ANALYSIS Support, Non-U.S. Gov't JOURNAL ARTICLEKWDadultantigens,cd45/immunologycytoplasm/chemistrycd4-positivet-lymphocytes/immunologycd8-positivet-lymphocytes/immunologyfemalefluorescentantibodytechniquehumanhivinfections/KWDgenetics/KWDimmunologylymphocytes/ultrastructuremalephosphotyrosine/analysissupport,non-uKWDsKWDgov'tjournalarticle
970228
M9721893

Copyright © 1997 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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