Selection of T cells reactive against autologous B lymphoblastoid cells during chronic rheumatoid arthritis.

Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

Selection of T cells reactive against autologous B lymphoblastoid cells during chronic rheumatoid arthritis.

J Immunol. 1996 Nov 15;157(10):4697-706. Unique Identifier : AIDSLINE MED/97064232
David-Ameline J; Lim A; Davodeau F; Peyrat MA; Berthelot JM; Semana G; Pannetier C; Gaschet J; Vie H; Even J; Bonneville M; INSERM Unit 211, Institute of Biology, Nantes, France.

Abstract: The repertoire and Ag specificity of T cells infiltrating inflamed joints from a chronic rheumatoid arthritis (RA) patient were studied in detail. Repertoire analysis demonstrated a reduced clonality of joint-infiltrating lymphocytes (JIL) as compared with patient's PBL, which was presumably due to an intra-articular expansion of T cell clones with recurrent TCR features. Strikingly, a large fraction of these JIL T cell clones, which were predominantly CD8+, proliferated in vitro when exposed to autologous B lymphoblastoid cells (BLC), unlike randomly chosen PBL clones derived from the same patient. This proliferative response was HLA-restricted, which confirmed a classical TCR-mediated recognition of BLC and was not observed against autologous PHA blasts, suggesting recognition of either EBV or B cell-specific Ags. Finally, a preliminary analysis of synovial lymphocytes derived from another chronic RA patient demonstrated a similar enrichment for T cells reactive against autologous BLC within JILs as compared with patient's PBLs. Taken together, these results, which suggest frequent expansions of autologous BLC-reactive T cells within inflamed joints of chronic RA patients, provide a basis for future studies evaluating the fine specificity and pathogenicity of these lymphocytes.

Keywords: Adult Arthritis, Rheumatoid/*IMMUNOLOGY/*PATHOLOGY B-Lymphocytes/*IMMUNOLOGY Cell Separation Chronic Disease Clone Cells CD8-Positive T-Lymphocytes/IMMUNOLOGY Female Flow Cytometry Human Joints *Lymphocyte Transformation Multigene Family/IMMUNOLOGY Receptors, Antigen, T-Cell, alpha-beta/IMMUNOLOGY Stem Cells/*IMMUNOLOGY Support, Non-U.S. Gov't T-Lymphocyte Subsets/*IMMUNOLOGY JOURNAL ARTICLE
970228
M9721857

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