Domains of the TCR beta-chain required for early thymocyte development. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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Domains of the TCR beta-chain required for early thymocyte development.

J Exp Med. 1996 Nov 1;184(5):1833-43. Unique Identifier : AIDSLINE MED/97079139
Jacobs H; Iacomini J; van de Ven M; Tonegawa S; Berns A; Division of Molecular Genetics, The Netherlands Cancer Institute,; Amsterdam, The Netherlands.

Abstract: The T cell receptor beta (TCR beta) chain controls the developmental transition from CD4-CD8- to CD4+8+thymocytes. We show that the extracellular constant region and the transmembrane region, but not the variable domain or cytoplasmic tail of the TCR beta chain are required for this differentiation step. TCR beta mutant chains lacking the cytoplasmic tail can be found at the cell surface both in functional TCR/CD3 complexes and in a GPI-anchored monomeric form indicating that the cytoplasmic tail of the TCR beta chain functions as an ER retention signal. The concordance between cell surface expression of the mutant chains as TCR/CD3 complexes and their capacity to mediate thymocyte differentiation supports the CD3 mediated feedback model in which preTCR/CD3 complexes control the developmental transition from CD4-CD8- to CD4+CD8+thymocytes.
Keywords: Animal Antigens, CD3/BIOSYNTHESIS Cell Compartmentation Cell Differentiation Cell Membrane/METABOLISM CD4-Positive T-Lymphocytes/*IMMUNOLOGY CD8-Positive T-Lymphocytes/*IMMUNOLOGY DNA Mutational Analysis Endoplasmic Reticulum/METABOLISM Glycosylphosphatidylinositols Mice Mice, Transgenic Models, Immunological Protein Conformation Receptors, Antigen, T-Cell, alpha-beta/GENETICS/*IMMUNOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transgenes JOURNAL ARTICLEKWDanimalantigens,cd3/biosynthesiscellcompartmentationcelldifferentiationcellmembrane/metabolismcd4-positivet-lymphocytes/KWDimmunologycd8-positivet-lymphocytes/KWDimmunologydnamutationalanalysisendoplasmicreticulum/metabolismglycosylphosphatidylinositolsmicemice,transgenicmodels,immunologicalproteinconformationreceptors,antigen,t-cell,alpha-beta/genetics/KWDimmunologysupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDtransgenesjournalarticle
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M9721845

Copyright © 1997 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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