Cloning and characterization of exodus, a novel beta-chemokine.

Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

Cloning and characterization of exodus, a novel beta-chemokine.

Blood. 1997 May 1;89(9):3315-22. Unique Identifier : AIDSLINE GENBANK/U64197
Hromas R; Gray PW; Chantry D; Godiska R; Krathwohl M; Fife K; Bell GI; Takeda J; Aronica S; Gordon M; Cooper S; Broxmeyer HE; Klemsz MJ; Department of Hematology/Oncology and the Walther Oncology; Center, Indiana University Medical Center, Indianapolis 46202,; USA.

Abstract: Chemokines are a family of related proteins that regulate leukocyte infiltration into inflamed tissue. Some chemokines such as MIP-1 alpha also inhibit hematopoietic progenitor cell proliferation. Recently, three chemokines, MIP-1 alpha, MIP-1 beta, and RANTES, have been found to significantly decrease human immunodeficiency virus production from infected T cells. We report here the cloning and characterization of a novel human chemokine termed Exodus for its chemotactic properties. This novel chemokine is distantly related to other chemokines (28% homology with MIP-1 alpha) and shares several biological activities. Exodus is expressed preferentially in lymphocytes and monocytes, and its expression is markedly upregulated by mediators of inflammation such as tumor necrosis factor or lipopolysaccharide. Purified synthetic Exodus was found to inhibit proliferation of myeloid progenitors in colony formation assays. Exodus also stimulated chemotaxis of peripheral blood mononuclear cells. The sequence homology, expression, and biological activity indicate that Exodus represents a novel divergent beta-chemokine.

Keywords: Amino Acid Sequence Base Sequence Blotting, Northern Bone Marrow/CYTOLOGY Cell Line Chemokines/*BIOSYNTHESIS/CHEMISTRY/PHARMACOLOGY Chemotaxis/DRUG EFFECTS Cloning, Molecular Comparative Study DNA, Complementary Gene Library Hematopoietic Stem Cells/CYTOLOGY/DRUG EFFECTS/*PHYSIOLOGY Human Islets of Langerhans/*METABOLISM Kinetics Molecular Sequence Data Organ Specificity Recombinant Proteins/BIOSYNTHESIS/PHARMACOLOGY Sequence Homology, Amino Acid Transcription, Genetic Tumor Cells, Cultured JOURNAL ARTICLE
970830
M9781072

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