Identification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells. NLM AIDSLINE Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

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Identification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells.

Blood. 1997 Jan 1;89(1):108-18. Unique Identifier : AIDSLINE MED/97132924
Hampson IN; Hampson L; Pinkoski M; Cross M; Heyworth CM; Bleackley RC; Atkinson E; Dexter TM; CRC Department of Experimental Haematology, Paterson Institute of; Cancer Research, Christie Hospital, Manchester, UK.

Abstract: We have identified a gene that has a high level of mRNA expression in undifferentiated, multipotential hematopoietic cells (FDCP-Mix) and that downregulates both transcript and protein, as these cells are induced to differentiate into mature myeloid cells. Sequence analysis of this gene has identified it as a serine protease inhibitor EB22/3 (serpin 2A). Constitutive expression of serpin 2A in FDCP-Mix cells was associated with an increase in the clonogenic potential of the cells and with a delay in the appearance of fully mature cells in cultures undergoing granulocyte macrophage differentiation when compared with control cells. Serpin 2A was also found to be expressed in bone marrow-derived bipotent granulocyte macrophage progenitor cells (GM-colony forming cell [CFC]), but not in erythrocyte progenitor cells from day 15 fetal liver. Expression of serpin 2A also showed a marked up regulation during the activation of cytotoxic suppressor CD8+ T cells, with a clear lag between the appearance of transcript and detection of protein.
Keywords: *CD8-Positive T-Lymphocytes/METABOLISM *Genes, Structural *Hematopoietic Stem Cells/METABOLISM *Lymphocyte Transformation *Serpins/ISOLATION & PURIFKWDcd8-positivet-lymphocytes/metabolismKWDgenes,structuralKWDhematopoieticstemcells/metabolismKWDlymphocytetransformationKWDserpins/isolation&purif
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