Determination of a HIV protease inhibitor (DMP 450) in animal and human plasma by solid-phase extraction and high-performance liquid chromatography.

Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

Determination of a HIV protease inhibitor (DMP 450) in animal and human plasma by solid-phase extraction and high-performance liquid chromatography.

J Chromatogr B Biomed Appl. 1996 Apr 12;678(2):303-8. Unique Identifier : AIDSLINE MED/96346984
Grubb MF; Robinson CA; White SJ; Krauthauser CL; Shum L; Drug Metabolism and Pharmacokinetics Section, DuPont Merck; Pharmaceutical Company, Stine-Haskell Research Center, Newark, DE; 19714, USA.

Abstract: Extraction of DMP 450 from plasma was performed with C2 solid-phase extraction columns, using 0.1 M ammonium acetate in 90% methanol to elute DMP 450. The extraction recovery over the range of 10 to 10 000 ng/ml averaged 81.0, 96.2, 77.4, 95.2 and 68.0% from rat, dog, monkey, chimpanzee (25-10 000 ng/ml) and human plasma, respectively. HPLC analysis was carried out with a C18 column and a mobile phase of acetonitrile, methanol and 30 mM potassium phosphate (pH 3), the composition dependent on the type of plasma being analyzed, and monitored at a wavelength of 229 nm. Intra-day and inter-day coefficients of variation were less than 9.9 and 12.9%, respectively. Absolute differences were less than 11.5%.

Keywords: *Azepines/BLOOD *Chromatography, High Pressure Liquid/METHODS *HIV Protease Inhibitors/BLOOD *Urea/ANALOGS & DERIVATIVES
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