Dendritic epidermal T-cell involvement in induction of CD8+ T cell-mediated immunity against an ultraviolet radiation-induced skin tumor.

Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

Dendritic epidermal T-cell involvement in induction of CD8+ T cell-mediated immunity against an ultraviolet radiation-induced skin tumor.

Int J Cancer. 1997 Jan 6;70(1):98-105. Unique Identifier : AIDSLINE MED/97138071
Cavanagh LL; Barnetson RS; Basten A; Halliday GM; Department of Medicine (Dermatology), University of Sydney at Royal; Prince Alfred Hospital, Camperdown, Australia. loisc@gpo.pa.uq.edu.au

Abstract: Murine epidermis contains 2 distinct cell populations which contribute to the skin immune system, Langerhans cells (LC), and dendritic epidermal T cells (DETC). LCs are important in the induction of immunity against a wide range of antigens; however, the function of DETC is unclear. To investigate the roles of these epidermal cells (EC) in protective antitumor immunity, an in vivo model of an ultraviolet radiation-induced fibrosarcoma, UV-13-1, was used. Mice were immunized with tumor antigen-pulsed EC followed 10 days later by an injection into the ear of 10(5) tumor cells, which did not lead to formation of a detectable tumor, but was intended to simulate the influence of a developing tumor on the ensuing immune response. The mice were then challenged with 2 x 10(6) viable tumor cells in each flank, sufficient to result in growth of a measurable tumor. Protective immunity was induced by DETC, and shown to be long-lasting, with tumors inoculated 160 days after immunization being effectively rejected. The effector cells responsible for protective immunity were CD8+ T cells. Delayed-type hypersensitivity generated by tumor antigen-pulsed EC was dependent on LCs, with no involvement of DETCs. This response, in contrast to that of DETC, required prior culture of EC with GM-CSF, but failed to inhibit tumor growth or incidence. Thus DETC and LC can both activate antitumor immune responses, although only the DETC-dependent response results in protective immunity in the presence of a developing tumor.

Keywords: *CD8-Positive T-Lymphocytes/IMMUNOLOGY *Dendritic Cells/IMMUNOLOGY *Neoplasms, Radiation-Induced/IMMUNOLOGY *Skin Neoplasms/IMMUNOLOGY *T-Lymphocytes/IMMUNOLOGY
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