NCp7 activates HIV-1Lai RNA dimerization by converting a transient loop-loop complex into a stable dimer.

Important note: Information in this article was accurate in 1997. The state of the art may have changed since the publication date.

NCp7 activates HIV-1Lai RNA dimerization by converting a transient loop-loop complex into a stable dimer.

J Biol Chem. 1996 Dec 27;271(52):33686-92. Unique Identifier : AIDSLINE MED/97126017
Muriaux D; De Rocquigny H; Roques BP; Paoletti J; Unite de Biochimie, URA 147 CNRS, 39 rue Camille Desmoulins, Institut; Gustave Roussy, 94805 Villejuif, France.

Abstract: Nucleocapsid protein 7 (NCp7), the human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein, was shown to strongly potentiate the dimerization of the retroviral genomic RNA. This process involves the interaction of two retroviral RNA monomer subunits near their 5'-ends. A region located upstream from the splice donor site was recently identified as being responsible for the formation of dimeric HIV-1 RNA. This region appeared to be confined within a stem-loop structure, with an autocomplementary sequence in the loop. In an in vitro study of spontaneous dimer formation, we reported that the 77-402 RNA transcript forms two distinct dimers differing in their thermostability: D37 and D55. We identified D37 as a "kissing" complex structure, formed via a loop-loop interaction between the two monomers, and D55 as a double stranded structure involving all nucleotides of the stem-loop via canonical base pairing. In this report, we have characterized the role of NCp7 in the HIV-1Lai RNA dimerization process by using in vitro dimerization assays with RNA transcripts of different lengths and dimer thermal dissociation. Our results show that the nucleocapsid protein NCp7 activates RNA dimerization very likely through interaction with the kissing complex and converts it into a stable dimer. Furthermore, this NCp7-promoted conversion only occurs if the 240-280 stem-loop structure is present in HIV-1Lai RNA molecules and contains the autocomplementary G257CGCGC262 sequence. This study suggests that, under physiological conditions, an NCp7-mediated RNA conformational change is involved in the maturation of the HIV-1 RNA dimer.

Keywords: *Capsid/PHARMACOLOGY *Gene Products, gag/PHARMACOLOGY *Nucleic Acid Conformation *RNA, Viral/METABOLISM *Zinc Fingers
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