CD4 Expression by erythroid precursor cells in human bone marrow.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

CD4 Expression by erythroid precursor cells in human bone marrow.

Blood. 1996 Mar 15;87(6):2275-82. Unique Identifier : AIDSLINE MED/96203792
Cleveland RP; Liu YC; Department of Pathology, Case Western Reserve University School; of Medicine, Cleveland, OH 44109 USA.

Abstract: Flow cytometry was used to assess CD4 expression in 62 consecutive bone marrow specimens from patients with a variety of clinical conditions. Using a lysed-whole-blood technique for labeling with monoclonal antibodies, two populations of CD4+ cells were identified within the lymphocyte/blast-cell fraction in 58 (94%) of these specimens. These consisted of (1) a population of T helper cells with high density expression of CD4 and (2) a second population of cells with low-density expression of CD4, which ranged from 1% to 36% of the gated cells. This latter population was present regardless of age, sex, or clinical condition including 21 of 21 specimens (100%) categorized as unremarkable bone marrows both morphologically and by flow cytometry and in four of four patients (100%) with human immunodeficiency virus-type 1 (HIV-1) infection. Coexpression of the erythroid lineage marker, glycophorin A, with the majority of cells in this second population was demonstrated in all 11 randomly selected samples using two-color flow cytometric analysis. These cells also expressed low levels of the myeloid markers, CD13 and CD33, but CD34 expression could not be demonstrated. These results provide evidence for expression of CD4 on cells of erythroid lineage in human marrow, and offer a potential mechanism for direct infection of erythroid precursor cells and deranged erythropoiesis in patients with HIV-1 infection.

Keywords: Adult Aged Aged, 80 and over Anemia/ETIOLOGY Antibodies, Monoclonal/IMMUNOLOGY Antigens, CD4/*BIOSYNTHESIS/GENETICS Biological Markers Bone Marrow/CYTOLOGY/*PATHOLOGY Child Child, Preschool CD4-Positive T-Lymphocytes/METABOLISM Erythroid Progenitor Cells/*METABOLISM Erythropoiesis Female Flow Cytometry Gene Expression Glycophorin/BIOSYNTHESIS Human HIV Infections/BLOOD/*PATHOLOGY Infant Leukemia/PATHOLOGY Lymphoma/PATHOLOGY Male Middle Age Neoplasms/PATHOLOGY T-Lymphocytes, Helper-Inducer/METABOLISM JOURNAL ARTICLE
960930
M9690942

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