IL-12-deficient mice are defective in IFN gamma production and type 1 cytokine responses. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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IL-12-deficient mice are defective in IFN gamma production and type 1 cytokine responses.

Immunity. 1996 May;4(5):471-81. Unique Identifier : AIDSLINE MED/96222810
Magram J; Connaughton SE; Warrier RR; Carvajal DM; Wu CY; Ferrante J; Stewart C; Sarmiento U; Faherty DA; Gately MK; Department of Biotechnology, Roche Institute of Molecular; Biology, Nutley, New Jersey 07110, USA.

Abstract: IL-12 is a cytokine that can exert regulatory effects on T and NK cells and promote Th1 responses. To delineate further the physiologic role of IL-12 in immunity, mice deficient for this cytokine were generated. IL-12-deficient mice were impaired but not completely lacking in the ability to produce IFN gamma following endotoxin administration and to mount a Th1 response in vivo, as measured by antigen-induced IFN gamma secretion by immune lymph node cells in vitro. In contrast, secretion of IL-4 was enhanced, while proliferation and secretion of IL-2 and IL-10 were normal following antigen stimulation. DTH responses were significantly reduced in IL-12-deficient mice, but no defect in allogeneic CTL responses was observed. These results indicate that IL-12 plays an essential role in regulating IFN gamma production and in facilitating normal DTH responses. However, other phenomena associated with Th1 responses and cell-mediated immunity, i.e., IL-2 secretion and CTL generation, were not compromised in the absence of IL-12.
Keywords: Animal Base Sequence Genetic Vectors/IMMUNOLOGY Interferon Type II/*BIOSYNTHESIS Interleukin-12/*DEFICIENCY/*GENETICS Killer Cells, Natural/IMMUNOLOGY Lymphocyte Transformation/GENETICS Lymphokines/*BIOSYNTHESIS Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Mutant Strains/GROWTH & DEVELOPMENT/*IMMUNOLOGY/METABOLISM Molecular Sequence Data Th1 Cells/IMMUNOLOGY/METABOLISM JOURNAL ARTICLEKWDanimalbasesequencegeneticvectors/immunologyinterferontypeii/KWDbiosynthesisinterleukin-12/KWDdeficiency/KWDgeneticskillercells,natural/immunologylymphocytetransformation/geneticslymphokines/KWDbiosynthesismicemice,inbredbalbcmice,inbredc57blmice,mutantstrains/growth&development/KWDimmunology/metabolismmolecularsequencedatath1cells/immunology/metabolismjournalarticle
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M9690931

Copyright © 1996 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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