Kaposi's sarcoma-associated herpesvirus (KSHV or HHV8) in primary effusion lymphoma: ultrastructural demonstration of herpesvirus in lymphoma cells.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Kaposi's sarcoma-associated herpesvirus (KSHV or HHV8) in primary effusion lymphoma: ultrastructural demonstration of herpesvirus in lymphoma cells.

Blood. 1996 Jun 15;87(12):4937-43. Unique Identifier : AIDSLINE MED/96247494
Said W; Chien K; Takeuchi S; Tasaka T; Asou H; Cho SK; de Vos S; Cesarman E; Knowles DM; Koeffler HP; Department of Pathology, Cedars Sinai Medical Center, Los; Angeles, CA 90048, USA.

Abstract: Recent molecular evidence suggests an association with a new herpesvirus, Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8), and primary effusion lymphomas (PELs). PELs have a characteristic morphology, phenotype, and clinical presentation, with malignant effusions in the absence of a contiguous solid tumor mass. We have established a cell line (KS-1) from a KSHV-positive human immunodeficiency virus (HIV)-negative patient with pleural cavity-based lymphoma that was passaged into triple-immunodeficient BNX mice. In contrast to cell lines from body cavity-based lymphomas derived from HIV-positive individuals that contain both KSHV and Epstein Barr viral genome, these cells contain only KSHV, allowing for uncontaminated virologic studies. Ultrastructural examination identified malignant cells with features of late differentiating B cells (immunoblasts). Cells with viral cytopathic effect contained typical 110-nm intranuclear herpesvirus nucleocapsids and complete cytoplasmic virions, confirming the association of PEL with KSHV.

Keywords: Animal B-Lymphocytes/ULTRASTRUCTURE/VIROLOGY Base Sequence Capsid/ULTRASTRUCTURE Cytopathogenic Effect, Viral DNA, Viral/ANALYSIS Herpesviridae Infections/*VIROLOGY Human Lymphoma, B-Cell/ULTRASTRUCTURE/*VIROLOGY Mice Mice, Nude Molecular Sequence Data Pleural Effusion/*VIROLOGY Pleural Neoplasms/ULTRASTRUCTURE/*VIROLOGY Polymerase Chain Reaction Rhadinovirus/*ISOLATION & PURIF/ULTRASTRUCTURE Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Tumor Cells, Cultured Tumor Stem Cells/ULTRASTRUCTURE/VIROLOGY JOURNAL ARTICLE
961030
M96A1415

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