Concordance between polymerase chain reaction and antibody detection in the diagnosis of human immunodeficiency virus type 1 infection. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Concordance between polymerase chain reaction and antibody detection in the diagnosis of human immunodeficiency virus type 1 infection.

Eur J Clin Microbiol Infect Dis. 1995 Nov;14(11):1011-14. Unique Identifier : AIDSLINE MED/96246490
Romano L; Catucci M; De Milito A; Venturi G; Zazzi M; Almi P; Gonnelli A; Rubino M; Maestrini R; Valensin PE; Dipartimento di Biologia Molecolare, Universita di Siena, Italy.

Abstract: A highly sensitive nested polymerase chain reaction (PCR) protocol was used to detect human immunodeficiency virus type 1 (HIV-1) DNA in peripheral blood mononuclear cells from 271 HIV-1-seropositive patients, 240 HIV-1-seronegative subjects at increased risk for HIV-1 infection, 51 serologically indeterminate individuals, and 120 healthy blood donors. PCR was carried out in a multiplex nested configuration with pol and env region primer sets. HIV-1 DNA was detected in all of the HIV-1 seropositive patients. In contrast, HIV-1 DNA was not detected in any of the either seronegative or serologically indeterminate subjects. Only one of 37 seronegative regular sexual partners of HIV-1-infected patients who were followed longitudinally was found to seroconvert to HIV-1. However, HIV-1 DNA and antibody results were concordant in the four samples obtained from this subject prior to and after seroconversion. These results show an excellent concordance between HIV-1 DNA and antibody detection for diagnosis of HIV-1 infection and suggest that long-term HIV-1 infection in the absence of detectable antibody is likely to occur at a very low frequency.
Keywords: Blotting, Western Comparative Study DNA, Viral/ANALYSIS Female Human HIV Antibodies/*ANALYSIS HIV Infections/*DIAGNOSIS HIV-1/*GENETICS/*IMMUNOLOGY Leukocytes, Mononuclear/CHEMISTRY Male *Polymerase Chain Reaction Serodiagnosis Support, Non-U.S. Gov't JOURNAL ARTICLEKWDblotting,westerncomparativestudydna,viral/analysisfemalehumanhivantibodies/KWDanalysishivinfections/KWDdiagnosishiv-1/KWDgenetics/KWDimmunologyleukocytes,mononuclear/chemistrymaleKWDpolymerasechainreactionserodiagnosissupport,non-uKWDsKWDgov'tjournalarticle
961030
M96A1375

Copyright © 1996 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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