Induction of single and dual cytotoxic T-lymphocyte responses to viral proteins in mice using recombinant hybrid Ty-virus-like particles.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Induction of single and dual cytotoxic T-lymphocyte responses to viral proteins in mice using recombinant hybrid Ty-virus-like particles.

Immunology. 1996 Feb;87(2):171-8. Unique Identifier : AIDSLINE MED/96245967
Layton GT; Harris SJ; Myhan J; West D; Gotch F; Hill-Perkins M; Cole JS; Meyers N; Woodrow S; French TJ; Adams SE; Kingsman AJ; British Biotech Pharmaceuticals Ltd, Oxford, UK.

Abstract: The induction of cytotoxic T-lymphocyte (CTL) responses to viral proteins is thought to be an essential component of protective immunity against viral infections. Methods for generating such responses in a reproducible manner would be of great value in vaccine development. We demonstrate here that the recombinant antigen-presentation system based on the yeast transposon (Ty) particle-forming p1 protein is a potent means of inducing CTL responses to a variety of viral CTL epitopes, including influenza virus nucleoprotein (two epitopes), Sendai virus and vesicular stomatitis virus nucleoproteins, and the V3 loop of human immunodeficiency virus type-1 (HIV-1) gp120. CTL were primed by hybrid Ty-virus-like particles (VLP) carrying the minimal epitope or as much as 19,000 MW of protein. Ty-VLP carrying two different epitopes (dual-epitope Ty-VLP) were capable of priming CTL responses in two different strains of mice or against two epitopes in the same individual. Furthermore, co-administration of a mixture of two different Ty-VLP carrying single epitopes could induce responses to both epitopes in the same individual. Ty-VLP appear to represent a reproducible and flexible system for inducing CTL responses in mice, and warrant further evaluation in primates.

Keywords: Amino Acid Sequence Animal Antigens, Viral/*IMMUNOLOGY Cytotoxicity, Immunologic DNA Insertion Elements/*IMMUNOLOGY Epitopes/IMMUNOLOGY Female HIV Envelope Protein gp120/*IMMUNOLOGY Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data Nucleoproteins/*IMMUNOLOGY Peptide Fragments/*IMMUNOLOGY Recombinant Proteins/IMMUNOLOGY T-Lymphocytes, Cytotoxic/*IMMUNOLOGY Viral Core Proteins/*IMMUNOLOGY JOURNAL ARTICLE
961130
M96B1815

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