Dynamic changes in circulating and antigen-responsive T-cell subpopulations post-Mycobacterium bovis infection in cattle.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Dynamic changes in circulating and antigen-responsive T-cell subpopulations post-Mycobacterium bovis infection in cattle.

Immunology. 1996 Feb;87(2):236-41. Unique Identifier : AIDSLINE MED/96245976
Pollock JM; Pollock DA; Campbell DG; Girvin RM; Crockard AD; Neill SD; Mackie DP; Veterinary Sciences Division, Department of Agriculture for; Northern Ireland, Stormont, Belfast.

Abstract: Bovine tuberculosis is a threat to animal and human health in several countries. Greater understanding of the immunology of the disease is required to develop improved tests and vaccines. This study has used a model of bovine tuberculosis, established in the natural host, to investigate the dynamic changes that occur in the circulating T-cell subpopulations after infection. When the phenotypic composition of the peripheral blood lymphocytes was determined pre- and post-experimental infection, the response to disease comprised three phases. Firstly, the WC1/gamma delta T cells decreased and then increased, suggesting localization to developing lesions and clonal expansion. Secondly, the CD4:CD8 ratio increased. Thirdly, the CD4:CD8 ratio decreased to less than pre-infection measurements. The latter changes suggested sequential involvement of CD4 and then CD8 T cells. The proportion of cells expressing interleukin-2 receptor (IL-2R) also increased. Panels of T-cell clones were established at various stages post-infection and all clones that exhibited antigen responsiveness were phenotyped. T-cell clones from early infection were WC1/gamma delta and CD4 in phenotype, while CD8 clones appeared later in infection, eventually becoming dominant. Therefore, from in vivo and in vitro evidence, it was suggested that there is a dynamic progression in the T-cell subpopulations involved dominantly in responses to mycobacteria.

Keywords: Animal Cattle Clone Cells/IMMUNOLOGY CD4-CD8 Ratio Flow Cytometry Immunophenotyping Kinetics Lymphocyte Transformation/IMMUNOLOGY Male Membrane Glycoproteins/ANALYSIS Receptors, Antigen, T-Cell, gamma-delta/ANALYSIS Support, Non-U.S. Gov't T-Lymphocyte Subsets/*IMMUNOLOGY Tuberculosis, Bovine/*IMMUNOLOGY JOURNAL ARTICLE
961130
M96B1811

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