Immunity to Cryptosporidium muris infection in mice is expressed through gut CD4+ intraepithelial lymphocytes.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Immunity to Cryptosporidium muris infection in mice is expressed through gut CD4+ intraepithelial lymphocytes.

Infect Immun. 1996 Jul;64(7):2556-62. Unique Identifier : AIDSLINE MED/96294764
McDonald V; Robinson HA; Kelly JP; Bancroft GJ; Department of Clinical Sciences, London School of Hygiene and; Tropical Medicine, London, United Kingdom.

Abstract: The role of gut intraepithelial lymphocytes (IEL) in immunity to cryptosporidial infection was investigated with a murine infection model involving Cryptosporidium muris. Oocyst shedding was monitored in severe combined immunodeficiency (SCID) mice infected with C. muris following intravenous injection of mesenteric lymph node (MLN) cells or intestinal IEL from BALB/c donor mice which were naive or previously infected with C. muris. SCID mice receiving no lymphoid cells developed chronic infections and excreted large numbers of oocysts until the end of the experiment. SCID mice injected with IEL from immune animals, however, were able to overcome the infection, and furthermore, these animals produced fewer oocysts and recovered sooner than ones which received IEL or MLN cells from naive BALB/c donors. Similar levels of protection were obtained in SCID mice injected with either 2 X 10(6) IEL or MLN cells from immune donor mice. Depletion of CD4+ cells from immune IEL, however, abrogated the ability to transfer immunity to SCID mice, while depletion of CD8+ cells only marginally reduced the protective capacity of immune IEL. Finally, control SCID mice which received no lymphocytes had < or = 1% CD4+ cells in the IEL from the small intestine, whereas the IEL from SCID mice recovered from infection, as a result of injection with immune IEL, contained 15% CD4+ cells. Thus, the ability to control C. muris infection correlated with the presence of the protective CD4+ cells in the gut epithelium.

Keywords: Animal Cell Movement Cryptosporidiosis/*IMMUNOLOGY/PATHOLOGY Cryptosporidium/IMMUNOLOGY CD4-Positive T-Lymphocytes/*IMMUNOLOGY/PATHOLOGY/PHYSIOLOGY CD8-Positive T-Lymphocytes/IMMUNOLOGY/PATHOLOGY Epithelium/IMMUNOLOGY/PATHOLOGY Female Immunocompetence Immunotherapy, Adoptive Intestinal Mucosa/*IMMUNOLOGY/PATHOLOGY Male Mice Mice, Inbred BALB C Mice, SCID Support, Non-U.S. Gov't JOURNAL ARTICLE
961130
M96B1806

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