Analysis and localization of cyclophilin A found in the virions of human immunodeficiency virus type 1 MN strain. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Analysis and localization of cyclophilin A found in the virions of human immunodeficiency virus type 1 MN strain.

AIDS Res Hum Retroviruses. 1995 Sep;11(9):1003-6. Unique Identifier : AIDSLINE MED/96089206
Ott DE; Coren LV; Johnson DG; Sowder RC 2nd; Arthur LO; Henderson LE; AIDS Vaccine Program, National Cancer Institute, Frederick Cancer; Research and Development Center, Maryland 21702-1201, USA.

Abstract: Previous reports have shown that cyclophilin A (CyPA) is found to be specifically associated with human immunodeficiency virus type-1 (HIV-1) virions and is required for infectivity (Franke et al. Nature 372:359; Thali et al. Nature 372:363). We have examined CyPA associated with HIV-1MN virions. Virions from infected human lymphoid cells were analyzed by high-pressure liquid chromatography (HPLC), protein sequence, and immunoblot analysis. At least three forms of CyPA were found: an unmodified form, an N-terminally modified form, and an N-terminally modified form that migrates as a larger isoform on a reducing-SDS polyacrylamide gel. Using a protease digestion procedure, CyPA that is associated with virions was found to be located inside the viral membrane. Similar examination of SIVMne produced by HUT-78 human T cells did not detect specific incorporation of CyPA into SIV virions. Our results are consistent with the role of CyPA acting early in the infectious process of HIV-1.
Keywords: Amino Acid Isomerases/*ANALYSIS/CHEMISTRY/METABOLISM Amino Acids/ANALYSIS Carrier Proteins/*ANALYSIS/CHEMISTRY/METABOLISM Cell Line Chromatography, High Pressure Liquid Human HIV-1/*CHEMISTRY/GROWTH & DEVELOPMENT/METABOLISM Immunoblotting Subtilisins Support, U.S. Gov't, P.H.S. Viral Envelope Proteins/ANALYSIS/METABOLISM JOURNAL ARTICLEKWDaminoacidisomerases/KWDanalysis/chemistry/metabolismaminoacids/analysiscarrierproteins/KWDanalysis/chemistry/metabolismcelllinechromatography,highpressureliquidhumanhiv-1/KWDchemistry/growth&development/metabolismimmunoblottingsubtilisinssupport,uKWDsKWDgov't,pKWDhKWDsKWDviralenvelopeproteins/analysis/metabolismjournalarticle
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