Enhanced susceptibility to human immunodeficiency virus infection in CD4+ T lymphocytes genetically deficient in CD43. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Enhanced susceptibility to human immunodeficiency virus infection in CD4+ T lymphocytes genetically deficient in CD43.

AIDS Res Hum Retroviruses. 1995 Sep;11(9):1015-21. Unique Identifier : AIDSLINE MED/96089208
Srinivas RV; Su T; Trimble LA; Lieberman J; Ardman B; Department of Infectious Diseases, St. Jude Children's Research; Hospital, Memphis, Tennessee 38105, USA.

Abstract: CD43 is a cell surface sialoglycoprotein expressed by most cells of hematopoietic origin, including all T lymphocytes. Elimination of CD43 expression by gene targeting in the CEM T cell line results in its increased homotypic adhesion and binding to HIV-1 gp120. Here we report that the CD43-negative CEM cells show increased susceptibility to HIV-1 infection and increased viral replication compared with the parental CD43+ CEM cell line. Increased HIV-1 replication also was observed in CEM cells with diminished CD43 expression secondary to functional inactivation of a single CD43 allele. The CD43- CEM cells were more susceptible to HIV-1-induced cytopathicity than their CD43+ counterparts. HIV-1 replication also was increased in the CD43- CEM cells after transfection with the infectious HIV molecular clone pNL4-3. These data suggest that factors that diminish CD43 expression on T lymphocytes may enhance their susceptibility to HIV-1 infection.
Keywords: Antigens, CD/GENETICS/*PHYSIOLOGY Cell Line Cell Survival Cytopathogenic Effect, Viral/IMMUNOLOGY CD4-Positive T-Lymphocytes/*IMMUNOLOGY/*VIROLOGY Gene Targeting Human HIV Infections/*ETIOLOGY/IMMUNOLOGY HIV-1/PHYSIOLOGY/*PATHOGENICITY Kinetics Sialoglycoproteins/*DEFICIENCY/GENETICS/IMMUNOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Virus Replication JOURNAL ARTICLEKWDantigens,cd/genetics/KWDphysiologycelllinecellsurvivalcytopathogeniceffect,viral/immunologycd4-positivet-lymphocytes/KWDimmunology/KWDvirologygenetargetinghumanhivinfections/KWDetiology/immunologyhiv-1/physiology/KWDpathogenicitykineticssialoglycoproteins/KWDdeficiency/genetics/immunologysupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDvirusreplicationjournalarticle
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