Major histocompatibility complex class I-restricted CD8+ T cells and class II-restricted CD4+ T cells, respectively, mediate and regulate contact sensitivity to dinitrofluorobenzene.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Major histocompatibility complex class I-restricted CD8+ T cells and class II-restricted CD4+ T cells, respectively, mediate and regulate contact sensitivity to dinitrofluorobenzene.

Eur J Immunol. 1995 Nov;25(11):3006-10. Unique Identifier : AIDSLINE MED/96085168
Bour H; Peyron E; Gaucherand M; Garrigue JL; Desvignes C; Kaiserlian D; Revillard JP; Nicolas JF; INSERM U.80, Universite Claude Bernard Lyon I, Faculte A.; Carrel, France.

Abstract: Contact sensitivity (CS) is a form of delayed-type hypersensitivity to haptens applied epicutaneously and is thought to be mediated, like classical delayed-type hypersensitivity responses, by CD4+ T helper-1 cells. The aim of this study was to identify the effector T cells involved in CS. We studied CS to the strongly sensitizing hapten dinitrofluorobenzene (DNFB) in mice rendered deficient by homologous recombination in either major histocompatibility complex (MHC) class I, MHC class II, or both, and which exhibited deficiencies in, respectively, CD8+, CD4+, or both, T cells. MHC class I single-deficient and MHC class I/class II double-deficient mice, both of which have a drastic reduction in the number of CD8+ T cells, were unable to mount a CS response to DNFB. In contrast, both MHC class II-deficient mice and normal mice treated with an anti-CD4 monoclonal antibody (mAb) developed exaggerated and persistent responses relative to heterozygous control littermates. Furthermore, anti-CD8 mAb depletion of class II-deficient mice totally abolished their ability to mount an inflammatory response to DNFB. Removal of residual CD4+ T cells in class II-deficient mice by anti-CD4 mAb treatment did not diminish the intensity of CS. These data clearly demonstrate that class I-restricted CD8+ T cells are sufficient for the induction of CS to DNFB, and further support the idea that MHC class II-restricted CD4+ T cells down-regulate this inflammatory response.

Keywords: Animal CD4-Positive T-Lymphocytes/*IMMUNOLOGY CD8-Positive T-Lymphocytes/*IMMUNOLOGY Dermatitis, Allergic Contact/*IMMUNOLOGY Dinitrofluorobenzene/*IMMUNOLOGY Down-Regulation (Physiology)/GENETICS Female Haptens/*IMMUNOLOGY Histocompatibility Antigens Class I/GENETICS Histocompatibility Antigens Class II/GENETICS Major Histocompatibility Complex/*GENETICS Male Mice Mice, Inbred C57BL Mice, Mutant Strains Support, Non-U.S. Gov't JOURNAL ARTICLE
960330
M9630718

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