Characterization of mouse nasal lymphocytes isolated by enzymatic extraction with collagenase.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Characterization of mouse nasal lymphocytes isolated by enzymatic extraction with collagenase.

J Immunol Methods. 1995 Nov 16;187(1):41-51. Unique Identifier : AIDSLINE MED/96085237
Asanuma H; Inaba Y; Aizawa C; Kurata T; Tamura S; Department of Pathology, National Institute of Health, Tokyo,; Japan.

Abstract: A reproducible method for isolation of mouse nasal lymphocytes was developed. The cells were released from tissue fragments of dissected mouse nose by enzyme extraction with collagenase and separated by a stepwise Percoll gradient centrifugation. The partially purified nasal lymphocyte fraction from normal BALB/c mice contained CD4+ T cells (18-23%), CD8+ T cells (7-10%) and B cells (20-38%), when analysed with a FACScan fluorescence analyser. The ratio of T to B cells and that of CD4+ to CD8+ T cells in the nasal cell fraction were about twice as high as those in Peyer's path lymphocytes. The nasal lymphocyte fraction from the mice infected with influenza virus was then characterized. The nasal lymphocytes contained a twice larger number of CD4+ and a three times larger number of CD8+ T cells than those of normal mice 7 days after infection. They produced IFN-gamma which increased after infection. They contained the cells secreting influenza virus-specific IgA and IgG antibodies 4 weeks after infection. Moreover, the nasal lymphocytes from infected C3H mice lysed the virus infected-target cells. These results suggest that this method can successfully be used for investigating cellular dynamics of mucosal immunity in the upper respiratory tract of experimental animals.

Keywords: Animal Antibodies, Viral/*ANALYSIS Cell Separation/METHODS Collagenases Cytotoxicity Tests, Immunologic CD4-CD8 Ratio Female IgA/ANALYSIS IgG/ANALYSIS Immunologic Techniques Influenza/*IMMUNOLOGY Interferon Type II/ANALYSIS Interleukin-4/ANALYSIS Lymphocyte Subsets/*IMMUNOLOGY Mice Mice, Inbred BALB C Mice, Inbred C3H Nasal Mucosa/*IMMUNOLOGY/VIROLOGY Orthomyxoviridae/*IMMUNOLOGY Peyer's Patches/IMMUNOLOGY JOURNAL ARTICLE
960330
M9630685

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