Type I insulin-like growth factor receptor expression in different developmental stages of human thymocytes. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Type I insulin-like growth factor receptor expression in different developmental stages of human thymocytes.

J Endocrinol. 1995 Nov;147(2):203-9. Unique Identifier : AIDSLINE MED/96096292
Kooijman R; Scholtens LE; Rijkers GT; Zegers BJ; Department of Immunology, University Hospital for Children and; Youth Het Wilhelmina Kinderziekenhuis, Utrecht, The Netherlands.

Abstract: Insulin-like growth factor-I (IGF-I) has been implicated in playing a regulatory role in T cell development and in T cell function. We demonstrate the presence of type I IGF receptors on human thymocytes using radioligand binding assays and flowcytometric analysis. The relative potencies of IGF-I, IGF-II and insulin for competition with 125I- IGF-I indicate the presence of type I IGF receptors. Scatchard analysis revealed that the average number of receptors per thymocyte is 257 +/- 28 with a Kd of 0.12 +/- 0.01. With multicolour flowcytometry using a type I IGF receptor specific monoclonal antibody (alpha IR3), we show that CD4-CD8- cells express 3-4 times more receptors per cell as compared with CD4+CD8+, CD4+CD8- and CD4-CD8+ cells. IGF-I directly stimulated DNA synthesis of thymocytes and potentiated DNA synthesis in mitogen-activated thymocytes. These results indicate that IGF-I can influence T cell development in humans at the level of the thymus.
Keywords: Binding, Competitive CD4-Positive T-Lymphocytes/METABOLISM CD8-Positive T-Lymphocytes/METABOLISM Cells, Cultured DNA/BIOSYNTHESIS Flow Cytometry Fluorescent Antibody Technique Human Insulin/METABOLISM Insulin-Like Growth Factor I/METABOLISM/PHARMACOLOGY Insulin-Like Growth Factor II/METABOLISM Radioligand Assay Receptors, Insulin-Like-Growth Factor I/*ANALYSIS/METABOLISM T-Lymphocytes/*METABOLISM/PHYSIOLOGY Thymus Gland/METABOLISM JOURNAL ARTICLE
960330
M9630682

Copyright © 1996 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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