Characterization of Epstein-Barr virus-infected cells in benign lymphadenopathy of patients seropositive for human immunodeficiency virus.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Characterization of Epstein-Barr virus-infected cells in benign lymphadenopathy of patients seropositive for human immunodeficiency virus.

Hum Pathol. 1996 Mar;27(3):263-8. Unique Identifier : AIDSLINE MED/96180298
Brousset P; Schlaifer D; Roda D; Massip P; Marchou B; Delsol G; Department of Pathology, Centre Hospitalier Universitaire de; Purpan, Toulouse, France.

Abstract: The authors investigated 25 benign lymph nodes in patients infected with the human immunodeficiency virus (HIV) by in situ hybridization (ISH) and immunohistochemistry (IHC) to detect and characterize the Epstein-Barr virus (EBV)-infected cells. After ISH, 22 lymph nodes were found to contain various numbers of Epstein-Barr-encoded RNA (EBER)-positive cells. Most of these cells were B cells. In six lymph nodes with numerous EBV-infected cells, EBNA2-positive/LMP1-positive lymphoblastoid cells were detected by IHC. Exceptional cells (in two specimens) were positively labeled with antigen-Z Epstein-Barr replicative activator (ZEBRA) antibody or BamHI Left Frame 1/Not I (BHLF1/Not I) probes, indicating that EBV replication is not enhanced in the lymphocytes. In normal conditions (healthy individuals), small lymphocytes that express a restricted pattern of viral genes do escape immune response, whereas lymphoblastoid cells do not. Thus, impaired immune system may account for the late proliferation of lymphoblastoid cells (Epstein-Barr nuclear antigen [EBNA]2positive/latent membrane protein [LMP]1 positive) in HIV-infected patients, and could explain why EBV-driven, acquired immunodeficiency syndrome (AIDS)-related, non-Hodgkin's lymphoma occur more frequently in patients with low CD4-positive T cells.

Keywords: Adult Aged Antigens, CD20/ANALYSIS Antigens, CD3/ANALYSIS Antigens, Viral/ANALYSIS AIDS-Related Complex/PATHOLOGY/*VIROLOGY DNA-Binding Proteins/ANALYSIS Female Gene Expression Herpesviridae Infections/PATHOLOGY/VIROLOGY Herpesvirus 4, Human/GENETICS/*ISOLATION & PURIF Human HIV/ISOLATION & PURIF HIV Infections/PATHOLOGY/*VIROLOGY HIV Seropositivity Immunohistochemistry Lymph Nodes/PATHOLOGY/*VIROLOGY Lymphocytes/PATHOLOGY/*VIROLOGY Male Middle Age Retrospective Studies RNA, Viral/ANALYSIS Support, Non-U.S. Gov't Tumor Virus Infections/PATHOLOGY/VIROLOGY Viral Matrix Proteins/ANALYSIS JOURNAL ARTICLE
960730
M9670505

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