Selection of RNA-binding peptides in vivo.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Selection of RNA-binding peptides in vivo.

Nature. 1996 Mar 14;380(6570):175-9. Unique Identifier : AIDSLINE MED/96176484
Harada K; Martin SS; Frankel AD; Department of Biochemistry and Biophysics, University of; California, San Francisco 94143-0448, USA.

Abstract: Many priniciples of sequence-specific DNA recognition have been established over the past decade, largely from structural studies of protein-DNA and drug-DNA complexes. On the basis of these principles, it has been possible to design or select variants of known structural motifs, including zinc-fingers and minor groove-binding drugs, that bind desired sequences. Here we describe a strategy, based on transcriptional termination in bacteria, to identify specific RNA-binding peptides using the arginine-rich RNA-binding motif as a framework. Peptides were isolated from two combinatorial libraries that bind tightly and specifically to the Rev response element of HIV. It appears that alpha-helical peptides resembling Rev were selected from one library whereas new peptides that probably do not form helices were selected from the other, suggesting that the arginine-rich motif may be a particularly versatile framework for recognizing RNA structures.

Keywords: Amino Acid Sequence Arginine/METABOLISM Base Sequence Cloning, Molecular Gene Library Gene Products, rev/GENETICS/METABOLISM Gene Products, tat/GENETICS/METABOLISM HIV/METABOLISM Molecular Sequence Data Oligodeoxyribonucleotides Protein Binding Protein Structure, Secondary RNA-Binding Proteins/*ANALYSIS/CHEMISTRY/GENETICS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Viral Regulatory Proteins/METABOLISM JOURNAL ARTICLE
960730
M9670502

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