Prolongation of rat kidney allograft survival by nematodes.

Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

Prolongation of rat kidney allograft survival by nematodes.

Transplantation. 1996 Jan 27;61(2):184-8. Unique Identifier : AIDSLINE MED/96166719
Ledingham DL; McAlister VC; Ehigiator HN; Giacomantonio C; Theal M; Lee TD; Department of Microbiology, Dalhousie University, Halifax, Nova; Scotia, Canada.

Abstract: Nippostrongylus infection strongly stimulates TH2 activity in vivo. Given the evidence of cross regulation between TH2 and TH1 cells, and the link between TH1 activity and graft rejection, we examined the effects of Nippostrongylus infection on the fate of kidney allografts in rats. Both prior Nippostrongylus infection and prior treatment with a soluble worm product significantly delayed kidney allograft rejection. Control graft rejection occurred at 9.7 +/- 1.2 days whereas grafts in Nippostrongylus- or worm extract-treated recipients lasted 32.7 +/- 11.3 days and 21.5 +/- 4.6 days, respectively. At day 5 posttransplant mononuclear cell infiltration was much reduced in the Nippostrongylus-treated recipients. Flow cytometry of isolated graft-infiltrating leukocytes showed a marked decrease in infiltrating T cells (82.8% reduction) with both CD4+ cells (81.0% reduction) and CD8+ cells (84.6% reduction) being reduced. CD8+ T cells, in particular, made up a much smaller proportion of the graft-infiltrating cells (22% rather than 49%) in the Nippostrongylus-treated animals as compared with untreated controls. Immunohistochemical assay of the graft tissue confirmed the flow cytometric results. Interleukin 4 expression was clearly demonstrated by RT-PCR of the isolated graft-infiltrating leukocytes from the Nippostrongylus-treated recipients but not from the control recipients. These data are consistent with our current hypothesis that Nippostrongylus delays graft rejection by inducing a cross-regulatory suppression of TH1 activity.

Keywords: Animal Base Sequence Graft Survival/*IMMUNOLOGY Interleukin-4/IMMUNOLOGY Kidney Transplantation/*IMMUNOLOGY Lymphocyte Transformation Molecular Sequence Data Nippostrongylus/*IMMUNOLOGY Rats Rats, Inbred BN Rats, Inbred Lew Strongylida Infections/*IMMUNOLOGY Support, Non-U.S. Gov't Th1 Cells/*IMMUNOLOGY Transplantation, Homologous JOURNAL ARTICLE
960730
M9670489

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