Lack of IL-4-induced Th2 response and IgE class switching in mice with disrupted Stat6 gene. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Lack of IL-4-induced Th2 response and IgE class switching in mice with disrupted Stat6 gene.

Nature. 1996 Apr 18;380(6575):630-3. Unique Identifier : AIDSLINE MED/96186813
Shimoda K; van Deursen J; Sangster MY; Sarawar SR; Carson RT; Tripp RA; Chu C; Quelle FW; Nosaka T; Vignali DA; Doherty PC; Grosveld G; Paul WE; Ihle JN; Department of Biochemistry, St Jude Children's Research Hospital,; Memphis, Tennessee 38105 USA.

Abstract: Signal transducers and activators of transcription (Stats) are activated by tyrosine phosphorylation in response to cytokines, and are thought to mediate many of their functional responses. Stat6 is activated in response to interleukin (IL)-4 and may contribute to various functions including mitogenesis, T-helper cell differentiation and immunoglobulin isotype switching. To evaluate the role of Stat6, we generated Stat6-null mice (Stat6 /-) by gene disruption in embryonic stem cells. The mice were viable, indicating the lack of a non-redundant function in normal development. Although naive lymphoid cell development was normal, Stat6 -/- mice were deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, and immunoglobulin class switching to IgE. In contrast, IL-4-mediated proliferation was only partly affected.
Keywords: Animal Base Sequence Cell Differentiation/GENETICS/PHYSIOLOGY Cell Line Cells, Cultured DNA Probes Gene Targeting IgE/*IMMUNOLOGY Immunity/GENETICS/PHYSIOLOGY *Immunoglobulin Class Switching Interleukin-4/*IMMUNOLOGY Lymphoid Tissue/IMMUNOLOGY Mice Molecular Sequence Data Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Th2 Cells/*CYTOLOGY/IMMUNOLOGY Trans-Activators/GENETICS/*PHYSIOLOGY JOURNAL ARTICLEKWDanimalbasesequencecelldifferentiation/genetics/physiologycelllinecells,cultureddnaprobesgenetargetingige/KWDimmunologyimmunity/genetics/physiologyKWDimmunoglobulinclassswitchinginterleukin-4/KWDimmunologylymphoidtissue/immunologymicemolecularsequencedatasupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDth2cells/KWDcytology/immunologytrans-activators/genetics/KWDphysiologyjournalarticle
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M9670425

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