Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
Localization of human immunodeficiency virus Rev in transfected and virus-infected cells.
AIDS Res Hum Retroviruses. 1995 Jul;11(7):795-804. Unique Identifier : AIDSLINE MED/96053842 Luznik L; Martone ME; Kraus G; Zhang Y; Xu Y; Ellisman MH; Wong-Staal F; Department of Medicine, UCSD School of Medicine, La Jolla,; California 92093-0665, USA.
Abstract:
The rev gene product of human immunodeficiency virus (HIV) is obligatory for viral replication. Rev interacts specifically with a structured RNA sequence within the viral genome termed the REV response element (RRE). Although the importance of Rev for the expression of viral proteins is well documented, its functional mechanism remains unresolved. Previous studies identified Rev in the absence of RRE to be a nuclear protein localized primarily within the nucleoli. To extend our understanding of the role of Rev in viral replication, immunolocalization studies of Rev and other nuclear components were carried out in transfected cells expressing both the Rev protein and RRE-containing mRNA and in cells infected with HIV. In both types of cells, Rev-like immunoreactivity was distributed both in the nucleoplasm and cytoplasm. Within the nucleus, Rev immunoreactivity was not evenly distributed but was present within focal concentrations. In transfected cells that were double labeled for Rev and SC-35, which labels a known component of spliceosomes, the foci of Rev labeling were distinct from the speckles labeled by SC-35, although Rev foci and speckles were often juxtaposed. In addition, morphological changes in the three-dimensional network of speckles were observed in both transfected cells expressing both the Rev protein and RRE-containing mRNA and in cells infected with HIV-1 and HIV-2. Our observations are consistent with the proposed dual role of Rev in mRNA transport and splicing.
Keywords: Base Sequence Cell Nucleolus/VIROLOGY DNA Primers Gene Expression Gene Products, rev/*ANALYSIS/BIOSYNTHESIS Genes, rev Genome, Viral Hela Cells Human HIV-2/GENETICS/*PHYSIOLOGY Molecular Sequence Data Polymerase Chain Reaction Recombinant Proteins/ANALYSIS/BIOSYNTHESIS Restriction Mapping RNA, Messenger/ANALYSIS/BIOSYNTHESIS Support, U.S. Gov't, P.H.S. Transfection Viral Proteins/BIOSYNTHESIS *Virus Replication JOURNAL ARTICLE
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Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
