Insulin-like growth factor 1 and insulin inhibit HIV type 1 replication in cultured cells. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Insulin-like growth factor 1 and insulin inhibit HIV type 1 replication in cultured cells.

AIDS Res Hum Retroviruses. 1995 May;11(5):555-61. Unique Identifier : AIDSLINE MED/96093890
Germinario RJ; DeSantis T; Wainberg MA; Lady Davis Institute for Medical Research, Sir Mortimer B.; Davis-Jewish General Hospital, Montreal, Quebec, Canada.

Abstract: Insulin-like growth factor 1 and insulin, considered primarily as metabolic and growth modulatory hormones, were found to inhibit the replication of HIV-1 in cultured cord blood mononuclear cells and chronically HIV-infected U937 cells. The effect of IGF-1 was seen at physiological concentrations or lower (1.7 x 10(-10) M) while that of insulin was observed at supraphysiological concentrations (8 x 10(-7) M). The EC50 for IGF-1 was found to be in the physiological range (2.5-4.5 x 10(-9) M) while that for insulin was considerably higher (1.1-3.3 x 10(-6) M). Insulin-like growth factor 1 and insulin at the concentrations employed exhibited no toxicity on the cells used in these studies. Furthermore, neither IGF-1 nor insulin exhibited any inhibitory activity on purified reverse transcriptase in vitro. Epidermal growth factor from 1.6 x 10(-10) to 1.6 x 10(-8) M demonstrated no inhibition of HIV-1 replication, while IGF-1 inhibited p24 antigen production 49 and 42% at 1.3 x 10(-9) and 1.3 x 10(-8) M IGF-1, respectively. These results suggest that IGF-1 under certain conditions has significant inhibitory effects on HIV-1 replication at physiological concentrations. This may prove to be of therapeutic value in patients infected with HIV-1.
Keywords: Cell Division/DRUG EFFECTS Cell Survival/DRUG EFFECTS Cells, Cultured Fetal Blood Human HIV-1/*DRUG EFFECTS/PHYSIOLOGY Insulin/*PHARMACOLOGY Insulin-Like Growth Factor I/*PHARMACOLOGY Leukocytes, Mononuclear/CYTOLOGY/VIROLOGY Support, Non-U.S. Gov't Virus Replication/*DRUG EFFECTS JOURNAL ARTICLEKWDcelldivision/drugeffectscellsurvival/drugeffectscells,culturedfetalbloodhumanhiv-1/KWDdrugeffects/physiologyinsulin/KWDpharmacologyinsulin-likegrowthfactori/KWDpharmacologyleukocytes,mononuclear/cytology/virologysupport,non-uKWDsKWDgov'tvirusreplication/KWDdrugeffectsjournalarticle
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M9621038

Copyright © 1996 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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