Molecular characterization of human immunodeficiency virus type 1 isolates from Venezuela. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Molecular characterization of human immunodeficiency virus type 1 isolates from Venezuela.

AIDS Res Hum Retroviruses. 1995 May;11(5):605-16. Unique Identifier : AIDSLINE GENBANK/U16779
Quinones-Mateu ME; Dopazo J; Este JA; Rota TR; Domingo E; Centro de Biologia Molecular Severo Ochoa (CSIC-UAM),; Universidad Autonoma de Madrid, Cantoblanco, Spain.

Abstract: Eight HIV-1 isolates from Venezuela have been characterized by nucleotide sequencing of the entire reverse transcriptase (RT)- and surface glycoprotein (gp 120)-coding regions. Average mutant frequencies were 2.5 x 10(-2) substitutions per nucleotide (s/nt) for the RT-coding region, and 10 x 10(-2) or 6.8 x 10(-2) s/nt for the gp120-coding region, depending on whether gaps introduced for optimal alignment were or were not, respectively, considered in the calculations. Phylogenetic trees were derived by maximum-likelihood, neighbor-joining, and maximum parsimony methods. In the trees derived from both RT- and gp120-coding regions, Venezuelan isolates cluster with subtype B viruses. However, the relative position of some of the isolates is considerably different in the two trees. Unique V3 loop amino acid sequences, not represented in the current database, have been identified among the Venezuelan isolates. In addition to representing the first molecular characterization of HIV-1 from Venezuela, the extensive genetic heterogeneity observed reinforces the interest in characterizing additional HIV-1 isolates worldwide for adequate vaccine design.
Keywords: Adult Amino Acid Sequence Base Sequence DNA Primers Genes, env Genes, pol Human HIV Envelope Protein gp120/*GENETICS HIV Infections/BLOOD/*VIROLOGY HIV-1/CLASSIFICATION/*GENETICS/ISOLATION & PURIF Male Middle Age Molecular Sequence Data Mutation Phylogeny RNA-Directed DNA Polymerase/*GENETICS Sequence Homology, Amino Acid Support, Non-U.S. Gov't Venezuela JOURNAL ARTICLEKWDadultaminoacidsequencebasesequencednaprimersgenes,envgenes,polhumanhivenvelopeproteingp120/KWDgeneticshivinfections/blood/KWDvirologyhiv-1/classification/KWDgenetics/isolation&purifmalemiddleagemolecularsequencedatamutationphylogenyrna-directeddnapolymerase/KWDgeneticssequencehomology,aminoacidsupport,non-uKWDsKWDgov'tvenezuelajournalarticle
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Copyright © 1996 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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