Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
Nucleotide sequence and restriction fragment length polymorphism analysis of the long terminal repeat of human T cell leukemia virus type II.
AIDS Res Hum Retroviruses. 1995 May;11(5):625-36. Unique Identifier : AIDSLINE GENBANK/L37146 Eiraku N; Monken C; Kubo T; Zhu SW; Rios M; Bianco C; Hjelle B; Nagashima K; Hall WW; Department of Medical Virology, Rockefeller University, New York,; New York 10021, USA.
Abstract:
Molecular studies have demonstrated the existence of two major subtypes of human T cell leukemia virus type II: HTLV-IIa and HTLV-IIb. In attempts to further classify this family of viruses we have carried out nucleotide sequence and restriction fragment length polymorphism (RFLP) analysis of the long terminal repeat (LTR), a region that has been shown in previous studies to have the greatest intra- and intersubtype genomic divergence. Analysis of the nucleotide sequences suggested the existence of distinct phylogenetic groups in each subtype and, on the basis of predicted differences in restriction endonuclease sites, RFLP analysis allowed the identification of four groups within the IIa subtype (a1-a4) and six within the IIb subtype (b1-b6). Nucleotide sequence analysis also suggested the possible existence of HTLV-II quasispecies. However, this appeared not to be significant, and preliminary studies suggest that these would not be expected to influence the results of RFLP analysis appreciably. The validity of the RFLP method was demonstrated in an analysis of 36 randomly chosen samples from HTLV-II seropositive blood donors from the New York City Blood Center, where it could be shown that all could be successfully classified. Moreover, the RFLP analysis correctly matched the viruses in donors and recipients of contaminated blood in four situations in which HTLV-II was inadvertently transmitted by transfusion. RFLP analysis of the LTR appears to be a rapid and reliable method by which to identify HTLV-II infection. This should prove useful in studies of the epidemiology and the characterization of viruses present both in nonindigenous and indigenous populations.
Keywords: Base Sequence DNA, Viral/GENETICS Human HTLV-BLV Infections/BLOOD/VIROLOGY HTLV-II/CLASSIFICATION/*GENETICS/ISOLATION & PURIF Indians, North American Molecular Sequence Data Phylogeny *Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid/*GENETICS RNA, Viral/GENETICS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE 960228
M9621030
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Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.
