Molecular cloning and characterization of a TAR-binding nuclear factor from T cells. NLM AIDSLINE Important note: Information in this article was accurate in 1996. The state of the art may have changed since the publication date.

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Molecular cloning and characterization of a TAR-binding nuclear factor from T cells.

AIDS Res Hum Retroviruses. 1995 Jun;11(6):663-9. Unique Identifier : AIDSLINE GENBANK/L22453
Reddy TR; Suhasini M; Rappaport J; Looney DJ; Kraus G; Wong-Staal F; Department of Medicine, University of California, San Diego, La; Jolla 92093-0665, USA.

Abstract: The TAt protein of the human immunodeficiency virus type 1 (HIV-1) activates the expression of viral mRNA through a cis-acting element in the LTR termed TAR. TAR RNA forms a stable stem-loop structure. Mutagenesis studies indicate that the stem structure, the primary sequence of the loop, and three unpaired bases in the stem (bulge) are important for Tat activation. Using the in vitro-transcribed TAR RNA as a probe, we have cloned a gene (TARBP-b) that encodes a TAR-binding protein from a cDNA expression library derived from Hut-78 cells. Expression of the 1.4-kb TARBP-b mRNA was observed in all mammalian cell lines tested. TARBP-b binds specifically to the bulge region of TAR RNA and trans-activates the HIV-1 long terminal repeat in the presence of ptat and prev expression plasmids. These results suggest that TARBP-b contributes to tat-mediated trans-activation.
Keywords: Amino Acid Sequence Animal Base Sequence Cell Line Gene Expression Regulation, Viral/GENETICS Gene Products, rev/PHYSIOLOGY Gene Products, tat/PHYSIOLOGY Genes, Viral/GENETICS Human HIV Long Terminal Repeat/GENETICS HIV-1/*GENETICS Lymphocytes/VIROLOGY Mice Molecular Sequence Data Nucleic Acid Conformation RNA Probes RNA-Binding Proteins/CHEMISTRY/*GENETICS RNA, Messenger/BIOSYNTHESIS RNA, Viral/CHEMISTRY/*METABOLISM Sequence Analysis, DNA Support, U.S. Gov't, P.H.S. T-Lymphocytes/*VIROLOGY Trans-Activation (Genetics)/*GENETICS JOURNAL ARTICLEKWDaminoacidsequenceanimalbasesequencecelllinegeneexpressionregulation,viral/geneticsgeneproducts,rev/physiologygeneproducts,tat/physiologygenes,viral/geneticshumanhivlongterminalrepeat/geneticshiv-1/KWDgeneticslymphocytes/virologymicemolecularsequencedatanucleicacidconformationrnaprobesrna-bindingproteins/chemistry/KWDgeneticsrna,messenger/biosynthesisrna,viral/chemistry/KWDmetabolismsequenceanalysis,dnasupport,uKWDsKWDgov't,pKWDhKWDsKWDt-lymphocytes/KWDvirologytrans-activation(genetics)/KWDgeneticsjournalarticle
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